SpringerProtocols: Abstract: The β-Glucuronidase (gus) Reporter Gene System: Gene Fusions; Spectrophotometric, Fluorometric, and Histochemical Detection

The β-Glucuronidase (gus) Reporter Gene System: Gene Fusions; Spectrophotometric, Fluorometric, and Histochemical Detection

By: Gillian A. Hull², Martine Devic²

Abstract

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In 1987, Richard Jefferson et al. (1) demonstrated the application of a new reporter gene system in transgenic plants. The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly referred to as the gus gene) has become one of the most widely used reporter genes in plant molecular biology. (For a detailed description of the gus gene, see Chapter 1.)

Affiliation(s): (2) Laboratoire de Physiologie et Biologie Moleculaire, Universite de Perpignan, France

Book Title: Plant Gene Transfer and Expression Protocols

Series: Methods in Molecular Biology | Volume: 49 | Pub. Date: Sep-27-1995 | Page Range: 125-141 | DOI: 10.1385/0-89603-321-X:125

Subject: Plant Sciences

References

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