SpringerProtocols: Abstract: Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues

Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues

By: Pilar Barcelo², Paul A. Lazzeri³

Abstract

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The cereals are one of the groups of crops more recalcitrant to transformation. Since, with the exception of rice (1), cereals have not been transformed by Agrobacterium, and highly regenerable protoplast systems are difficult to obtain, these species have remained untransformed for a much longer time than the major dicot species. It was not until 1988 and 1989 that the first transgenic cereal plants (maize [2]; rice [3]) were produced by the use of direct DNA-transfer into protoplasts. This method, however, has not yet given rise to transgenic wheat or barley plants, species in which the protoplast systems established are not as regenerative and stable as those developed for rice or maize.

Affiliation(s): (2) Biochemistry and Physiology Department, Institute of Arable Crops Research, Harpenden, UK
(3) Biochemistry and Physiology Department, AFRC Institute of Arable Crops Research, Harpenden, UK

Book Title: Plant Gene Transfer and Expression Protocols

Series: Methods in Molecular Biology | Volume: 49 | Pub. Date: Sep-27-1995 | Page Range: 113-123 | DOI: 10.1385/0-89603-321-X:113

Subject: Plant Sciences

References

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