11. Isolation of Yeast DNA
The problems often experienced with DNA isolated from yeast are mostly owing to the impurities remaining in DNA isolated according
to certain isolation protocols. Incomplete cutting of chromosomal DNA with restriction endonucleases, or poor transformation
of Escherichia coli with plasmid DNA from yeast are the difficulties most frequently encountered. The procedures given herein have been chosen
since they have used successfully by many workers to obtain DNA that does not give these problems.
Affiliation(s): (2) Department of Biochemistry and Molecular Biology, University College London, UK Series: Methods in Molecular Biology | Volume: 53 | Pub. Date: Feb-19-1996 | Page Range: 103-107 | DOI: 10.1385/0-89603-319-8:103
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