SpringerProtocols: Abstract: Micellar Electrokinetic Chromatography

Micellar Electrokinetic Chromatography

By: Koji Otsuka^{2 3}, Shigeru Terabe³

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Recently, capillary electrophoresis (CE) or high-performance capillary electrophoresis (HPCE) has become popular as a high-resolution separation method. The technique was first introduced by Mikkers et al. (1), Jorgenson and Lukacs (2), and HjertÃ©n (3), as an instrumental version of electrophoresis. Although CE can generally give a higher resolution within a shorter time compared with conventional high-performance liquid chromatography (HPLC), only ionic or charged solutes can be separated by CE, in principle. This was a serious limitation of CE, but the development of electrokinetic chromatography (EKC) (4) has been able to solve such problems. EKC is based on chromatographic principles using homogeneous solutions containing an ionic “carrier” and the same apparatus as CE. The unique characteristic of EKC is that both neutral and charged analytes can be separated electrophoretically. Among various modes of EKC, micellar EKC (MEKC) (5–7), which uses micellar solutions of ionic surfactants, has become the most popular technique for the separation of small neutral molecules.

Affiliation(s): (2) Department of Industrial Chemistry, Osaka Prefectural College of Technology, Meyagawa, Osaka, Japan
(3) Department of Material Science, Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo, Japan

Book Title: Capillary Electrophoresis Guidebook: Principles, Operation, and Applications

Series: Methods in Molecular Biology | Volume: 52 | Pub. Date: Nov-02-1995 | Page Range: 125-155 | DOI: 10.1385/0-89603-315-5:125

Subject: Biochemistry

References

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1.	Mikkers, F. E. P., Everaerts, F. M., and Verheggen, Th. P. E. M. (1979) High-performance zone electrophoresis. J. Chromatogr. 169, 11–20.

2.	Jorgenson, J. W. and Lukacs, K. D. (1981) Zone electrophoresis in open-tubular glass capillaries. Anal. Chem. 53, 1298–1302.

3.	HjertÃ©n, S. (1983) High-performance electrophoresis: the electrophoretic counterpart of high-performance liquid chromatography. J. Chromatogr. 270, 1–6.

4.	Terabe, S. (1989) Electrokinetic chromatography: an interface between electrophoresis and chromatography. Trends Anal. Chem. 8, 129–134.

5.	Terabe, S., Otsuka, K., Ichikawa, K., Tsuchiya, A., and Ando, T. (1984) Electrokinetic separations with micellar solutions and open-tubular capillaries. Anal. Chem. 56, 111–113.

6.	Terabe, S., Otsuka, K., and Ando, T. (1985) Electrokinetic chromatography with micellar solution and open-tubular capillary. Anal. Chem. 57, 834–841.

7.	Terabe, S., Otsuka, K., and Ando, T. (1989) Band broadening in electrokinetic chromatography with micellar solutions and open-tubular capillaries. Anal. Chem. 61, 251–260.

8.	Kuhr, W. G. and Monnig, C. A. (1992) Capillary electrophoresis. Anal. Chem. 64, 389R–407R.

9.	Janini, G. M. and Issaq, H. J. (1992) Micellar electrokinetic capillary chromatography: basic considerations and current trends. J. Liquid Chromatogr. 15, 927–960.

10.	Vindevogel, J. and Sandra, P. (1992) Introduction to Micellar Electrokinetic Chromatography. Huthig, Heidelberg.

11.	Terabe, S. (1992) Micellar Electrokinetic Chromatography. Beckman, California.

12.	Terabe, S. (1993) Micellar electrokinetic chromatography, in Capillary Electrophoresis: Theory, Methodology, and Applications. (Guzman, N. A., ed.), Marcel Dekker, New York, pp. 65–87.

13.	Otsuka, K. and Terabe, S. (1989) Effects of pH on electrokinetic velocities in micellar electrokinetic chromatography. J. Microcol. Sep. 1, 150–154.

14.	Otsuka, K., Terabe, S., and Ando, T. (1985) Electrokinetic chromatography with micellar solutions: separation of phenylthiohydantoin-amino acids. J. Chromatogr. 312, 219–226.

15.	Nishi, H., Tsumagari, N., and Terabe, S. (1989) Effect of tetraalkylammonium salts on micellar electrokinetic chromatography of ionic substances. Anal. Chem. 61, 2434–2439.

16.	Terabe, S., Katsura, T., Okada, Y., Ishihama, Y., and Otsuka, K. (1993) Measurement of thermodynamic quantities of micellar solubilization by micellar electrokinetic chromatography with sodium dodecyl sulfate. J. Microcol. Sep. 5, 23–33.

17.	Ishihama, Y. and Terabe, S., unpublished data.

18.	Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1990) Effect of surfactant structures on the separation of cold medicine ingredients by micellar electrokinetic chromatography. J. Pharmaceut. Sci. 79, 519–523.

19.	Cole, R. O., Sepaniak, M. J., Hinze, W. C., Gorse, J., and Oldiges, K. (1991) Bile salt surfactants in micellar electrokinetic capillary chromatography. application to hydrophobic molecule separations. J. Chromatogr. 557, 113–123.

20.	Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1990) Separation and determination of lipophilic corticosteroids and benzothiazepin analogues by micellar electrokinetic chromatography using bile salts. J. Chromatogr. 513, 279–295.

21.	Terabe, S., Shibata, M., and Miyashita, Y. (1989) Chiral separation by electrokinetic chromatography with bile salt micelles. J. Chromatogr. 480, 403–411.

22.	Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1990) Chiral separation of diltiazem, trimetoquinol and related compounds by micellar electrokinetic chromatography. J. Chromatogr. 515, 233–243.

23.	Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1990) Chiral separation of trimetoquinol hydrochloride and related compounds by micellar electrokinetic chromatography using sodium taurodeoxycholate solutions and application to optical purity determination. Anal. Chim. Acta. 236, 281–286.

24.	Rasmussen, H. T., Goebel, L. K., and McNair, H. M. (1991) Optimization of resolution in micellar electrokinetic chromatography. J. High Resolut. Chromatogr. 14, 25–28.

25.	Otsuka, K., Terabe, S., and Ando, T. (1985) Electrokinetic chromatography with micellar solutions retention behaviour and separation of chlorinated phenols. J. Chromatogr. 348, 39–47.

26.	Terabe, S., Miyashita, Y., Shibata, O., Barnhart, E. R., Alexander, L. R., Patterson, D. G., Karger, B. L., Hosoya, K., and Tanaka, N. (1990) Separation of highly hydrophobic compounds by cyclodextrin-modified micellar electrokinetic chromatography. J. Chromatogr. 516, 23–31.

27.	Terabe, S., Ishihama, Y., Nishi, H., Fukuyama, T., and Otsuka, K. (1991) Effect of urea addition in micellar electrokinetic chromatography. J. Chromatogr. 545, 359–368.

28.	Otsuka, K. and Terabe, S. (1990) Effects of methanol and urea on optical resolution of phenylthiohydantoin-DL-amino acids by micellar electrokinetic chromatography with sodium N-dodecanoyl-L-valinate. Electrophoresis 11, 982–984.

29.	Otsuka, K., Terabe, S., and Ando, T. (1986) Separation of aromatic sulfides by electrokinetic chromatography with micellar solution (in Japanese). Nippon Kagaku Kaishi, 950–955.

30.	Gorse, J., Balchunas, A. T., Swaile, D. F., and Sepaniak, M. J. (1988) Effects of organic mobile phase modifiers in micellar electrokinetic capillary chromatography. J. High Resolut. Chromatogr. Chromatogr. Commun. 11, 554–559.

31.	Bushey, M. M. and Jorgenson, J. W. (1989) Separation of dansylated methylamine and dansylated methyl-d3-amine by micellar electrokinetic capillary chromatography with methanol-modified phase. Anal. Chem. 61, 491–493.

32.	Bushey, M. M. and Jorgenson, J. W. (1989) Effects of methanol-modified mobile phase on the separation of isotopically substituted compounds by micellar electrokinetic capillary chromatography. J. Microcol. Sep. 1, 125–130.

33.	Balchunas, A. T. and Sepaniak, M. J. (1988) Gradient elution for micellar electrokinetic capillary chromatography. Anal. Chem. 60, 617–621.

34.	Tanaka, N., Fukutome, T., Tanigawa, T., Hosoya, K., Kimata, K., Araki, T., and Unger, K. K. (1995) Structural selectivity provided by starburst dendrimers as pseudostationary phase in EKC. J. Chromatogr. A 699, 331–341.

35.	Kaneta, T., Yamashita, T., and Imasaka, T. (1992) Separation of polyaromatic hydrocarbons by laser-induced fluorescence detection—micellar electrokinetic chromatography, Abstracts of Papers, 12th Symposium on Capillary Electrophoresis, Himeji, Hyogo, Japan, Abstract 8.

36.	Otsuka, K., Koike, R., and Terabe, S. (1993) Effect of addition of organic modifiers in micellar electrokinetic chromatography, Abstracts of Papers, 65th Meeting of the Chemical Society of Japan, Tokyo, Japan; Abstract 3A252.

37.	Cohen, A. S., Terabe, S., Smith, J. A., and Karger, B. L. (1987) High-performance capillary electrophoretic separation of bases, nucleosides, and oligonucleotides retention manipulation via micellar solutions and metal additives. Anal. Chem. 59, 1021–1027.

38.	Nishi, H. and Terabe, S. (1990) Applications of micellar electrokinetic chromatography to pharmaceutical analysis. Electrophoresis 11, 691–701.

39.	Otsuka, K. and Terabe, S. (1993) Enantiomeric separation by micellar electrokinetic chromatography. Trends Anal. Chem. 12, 125–130.

40.	Otsuka, K. and Terabe, S. (1993) Chiral separation by capillary electrophoresis and electrokinetic chromatography, in Capillary Electrophoresis: Theory, Methodology, and Applications (Guzman, N. A., ed.), Marcel Dekker, New York, pp 617–629.

41.	Terabe, S., Otsuka, K., and Nishi, H. (1994) Separation of enantiomers by capillary electrophoretic techniques. J. Chromatogr. A 666, 295–319.

42.	Dobashi, A., Ono, T., Hara, S., and Yamaguchi, J. (1989) Optical resolution of enantiomers with chiral mixed micelles by electrokinetic chromatography. Anal. Chem. 61, 1984–1986.

43.	Dobashi, A., Ono, T., Hara, S., and Yamaguchi, J. (1989) Enantioselective hydrophobic entanglement of enantiomeric solutes with chiral functionalized micelles by electrokinetic chromatography. J. Chromatogr. 480, 413–420.

44.	Otsuka, K. and Terabe, S. (1990) Enantiomeric resolution by micellar electrokinetic chromatography with chiral surfactants. J. Chromatogr. 515, 221–226.

45.	Otsuka, K., Kawahara, J., Tatekawa, K., and Terabe, S. (1991) Chiral separations by micellar electrokinetic chromatography with sodium N-dodecanoyl-L-valinate. J. Chromatogr. 559, 209–214.

46.	Otsuka, K., Kashihara, M., Kawaguchi, Y., Koike, R., Hisamitsu, T., and Terabe, S. (1993) Optical resolution by high performance capillary electrophoresis: micellar electrokinetic chromatography with sodium N-dodecanoyl-L-glutamate and digitonin. J. Chromatogr. A 652, 253–257.

47.	Nishi, H., Fukuyama, T., Matsuo, M., and Terabe, S. (1989) Chiral separation of optical isomeric drugs using micellar electrokinetic chromatography and bile salts. J. Microcol. Sep. 1, 234–241.

48.	Cole, R. O., Sepaniak, M. J., and Hinze, W. L. (1990) Optimization of binaphtyl enantiomer separations by CZE using mobile phases containing bile salts and organic solvent. J. High Resolut. Chromatogr. 13, 579–582.

49.	Ishihama, Y. and Terabe, S. (1993) Enantiomeric separation by micellar electrokinetic chromatography using saponins. J. Liquid Chromatogr. 16, 933–944.

50.	Miyashita, Y. and Terabe, S. (1990) Separation of dansyl DL-amino acids by micellar electrokinetic capillary chromatography with and without cyclodextrins using P/ACE system 2000. Application Data, High Performance Capillary Electrophoresis, Beckman, DS-767.

51.	Nishi, H., Fukuyama, T., and Terabe, S. (1991) Chiral separation by cyclodextrin-modified micellar electrokinetic chromatography. J. Chromatogr. 553, 503–516.

52.	Ueda, T., Kitamura, F., Mitchell, R., Metcalf, T., Kuwana, T., and Nakamoto, A. (1991) Chiral separation of naphthalene-2,3-dicarboxaldehyde-labeled amino acid enantiomers by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. Anal. Chem. 63, 2979–2981.

53.	Otsuka, K. and Terabe, S. (1993) Optical resolution of chlorpheniramine by cyclodextrin added capillary zone electrophoresis and cyclodextrin modified micellar electrokinetic chromatography. J. Liquid Chromatogr. 16, 945–953.

54.	Foley, J. P. (1990) Optimization of micellar electrokinetic chromatography. Anal. Chem. 62, 1302–1308.

55.	Vindevogel, J. and Sandra, P. (1991) Resolution optimization in micellar electrokinetic chromatography use of Plackett-Burman statistical design for the analysis of testosterone ester. Anal. Chem. 63, 1530–1536.

56.	Strasters, J. K. and Khaledi, M. G. (1991) Migration behavior of cationic solutes in micellar electrokinetic capillary chromatography. Anal. Chem. 63, 2503–2508.

57.	Khaledi, M. G., Smith, S. C., and Strasters, J. K. (1991) Micellar electrokinetic capillary chromatography of acidic solutes: migration behavior and optimization strategies. Anal. Chem. 63, 1820–1830.

58.	Terabe, S., Ozaki, H., Otsuka, K., and Ando, T. (1985) Electrokinetic chromatography with 2-O-carboxymethyl-β-cyclodextrin as a moving “stationary” phase. J. Chromatogr. 332, 211–217.

59.	Terabe, S. and Isemura, T. (1990) Ion-exchange electrokinetic chromatography with polymer ions for the separation of isomeric ions having identical electrophoretic mobilities. Anal. Chem. 62, 650–652.

60.	Watarai, H. (1991) Microemulsion capillary electrophoresis. Chem. Lett. 391–394.

61.	Terabe, S., Matsubara, N., Ishihama, Y., and Okada, Y. (1992) Microemulsion electrokinetic chromatography: comparison with micellar electrokinetic chromatography. J. Chromatogr. 608, 23–29.

62.	Terabe, S., Ozaki, H., and Tanaka, Y. (1994) New pseudo-stationary phases for electrokinetic chromatography: a high-molecular surfactant and proteins. J. Chin. Chem. Soc. 41, 251–257.

63.	Ozaki, H., Ichihara, A., and Terabe, S. (1994) Micellar electrokinetic chromatography using high-molecular surfactants: use of butyl acrylate/butyl methacrylate/methacrylic acid copolymers sodium salts as pseudo-stationary phases. J. Chromatogr. A 680, 117–123.

64.	Ozaki, H., Ito, N., Terabe, S., Takada, Y., Sakairi, M., and Koizumi, H. (1995) Micellar electrokinetic chromatography-mass spectrometry (MEKC-MS) using a high-molecular surfactant: on-line coupling with an electrospray ionization interface. J. Chromatogr. A, in press.

65.	Ozaki, H., Ito, N., Terabe, S., Takada, Y., Sakairi, M., and Koizumi, H. (1995) MEKC-MS using high-molecular surfactants. Abstracts of Papers, 56th Forum of Analytical Chemistry, Osaka, Japan, Abstract 1D01.

66.	Terabe, S., Miyashita, Y., Ishihama, Y., and Shibata, O. (1993) Cyclodextrin-modified micellar electrokinetic chromatography. separation of hydrophobic and enantiomeric compounds. J. Chromatogr. 636, 47–55.

67.	Otsuka, K., Higashimori, M., Koike, R., Karuhaka, K., Okada, Y., and Terabe, S. (1994) Separation of lipophilic compounds by micellar electrokinetic chromatography with organic modifiers. Electrophoresis 15, 1280–1283.

68.	Tanaka, N., Tanigawa, T., Hosoya, K., Kimata, K., Araki, T., and Terabe, S. (1992) Starburst dendrimers as carriers in electrokinetic chromatography. Chem. Lett. 959–962.

69.	Tanaka, N., Fukutome, T., Hosoya, K., Kimata, K., and Araki, T. (1995) Polymer-supported pseudostationary phase for EKC. EKC in a full range of methanol-water mixtures with alkylated starburst dendrimers. J. Chromatogr., in press.

70.	Sahota, R. S. and Khaledi, M. G. (1994) Nonaqueous capillary electrophoresis. Anal. Chem. 66, 1141–1146.

71.	Ahuja, E. S. and Foley, J. P. (1994) Separation of very hydrophobic compounds by hydrophobic interaction electrokinetic chromatography. J. Chromatogr. A 680, 73–83.

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