SpringerProtocols: Abstract: 2. YAC Library Storage and Transport

2. YAC Library Storage and Transport

By: John E. Collins², Sheila Hassock³, Ian Dunham²

Abstract

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The large size of mammalian genomes necessitates the use of cloning vectors that will accommodate genomic DNA inserts of at least several hundred kilobases. The development of the yeast artificial chromosome (YAC) cloning system (1) in the mid-1980s and the construction of YAC libraries with large numbers of genome equivalents for both the human (2–5) and mouse genomes (5,6) provided a major impetus to mammalian genome mapping. These technical advances enabled the mapping of megabase-sized chromosomal regions (7,8), culminating in the first complete clone maps of single mammalian chromosomes (9,10). In 1994, it is fair to say that the success of any long range mapping and cloning project depends on access to YAC resources.

Affiliation(s): (2) Sanger Centre, Cambridge, UK
(3) Paediatric Research Unit, United Medical and Dental Schools of Guy’s and St. Thomas’ Hospitals, London, UK

Book Title: YAC Protocols

Series: Methods in Molecular Biology | Volume: 54 | Pub. Date: Oct-23-1995 | Page Range: 13-21 | DOI: 10.1385/0-89603-313-9:13

Subject: Genetics/Genomics

References

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