SpringerProtocols: Abstract: Gene Activation by T-DNA Tagging

Gene Activation by T-DNA Tagging

By: Klaus Fritze³, Richard Walden³

Abstract

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Agrobacterium-mediated transformation of plants has not only become a useful tool for the introduction of chimeric genes into plants, but it has also recently been utilized in gene tagging (for reviews, see refs. 1–3). T-DNA tagging has proven a powerful genetic approach for the isolation of novel plant genes. Compared with other approaches to gene isolation (for a review, see ref. 4), mutagenesis by insertion of defined DNA sequences allows the rapid cloning and characterization of the mutated locus. Furthermore, T-DNA is an ideal gene tag, as it has the potential to insert preferentially into potentially transcribed regions of the genome (5).

Affiliation(s): (3) Max Planck Institut fur Zuchtungsforschung, Koln, Germany

Book Title: Agrobacterium Protocols

Series: Methods in Molecular Biology | Volume: 44 | Pub. Date: Jun-01-1995 | Page Range: 281-294 | DOI: 10.1385/0-89603-302-3:281

Subject: Plant Sciences

References

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