SpringerProtocols: Abstract: Manipulating Photosynthesis in Transgenic Plants

Manipulating Photosynthesis in Transgenic Plants

By: Jacqueline S. Knight³, Francisco MadueÃ±o³, Simon A. Barnes³, John C. Gray³

Abstract

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Agrobacterium tumefaciens-mediated transformation of plants provides a relatively straightforward means of altering the amounts of individual proteins involved in photosynthetic processes. By the introduction of additional copies of genes or of chimeric cDNA constructs, it is possible to produce transgenic plants with increased or decreased amounts of specific photosynthetic components. These plants can then be analyzed to examine the effects of the altered protein levels on a wide range of physiological or biochemical processes.

Affiliation(s): (3) Department of Plant Sciences, University of Cambridge, UK

Book Title: Agrobacterium Protocols

Series: Methods in Molecular Biology | Volume: 44 | Pub. Date: Jun-01-1995 | Page Range: 263-280 | DOI: 10.1385/0-89603-302-3:263

Subject: Plant Sciences

References

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1.	Last, D. I. and Gray, J. C. (1990) Synthesis and accumulation of pea plastocyanin in transgenic tobacco plants. Plant Mol. Biol. 14, 229–238.

2.	Gray, J. C., Last, D. I., Dupree, P., Newman, B. J., and Slatter, R. E. (1990) Expression of genes for photosynthetic electron transfer components in transgenic plants, in Genetic Engineering of Crop Plants (Lycett, G. W. and Grierson, D., eds.), Butterworths, London, pp. 191–205.

3.	Napoli, C., Lemieux, C., and Jorgensen, R. (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2, 279–289.

4.	van der Krol, A. R., Mur, L. A., Beld, M., Mol, J. N. M., and Stuitje, A. R. (1990) Flavonoid genes in petunia addition of a limited number of gene copies may lead to suppression of gene expression. Plant Cell 2, 291–299.

5.	van der Krol, A. R., Lenting, P. E., Veenstra, J., van der Meer, I. M., Koes, R. E., Gerats, A. G. M., et al. (1988) An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333, 866–869.

6.	Smith, C. J. S., Watson, C. F., Ray, J., Bird, C. R., Morris, P. C., Schuch, W., and Grierson, D. (1988) Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334, 724–726.

7.	Rodermel, S. R., Abbott, M. S., and Bogorad, L. (1988) Nuclear-organelle interactions: nuclear antisense gene inhibits ribulose bisphosphate carboxylase enzyme levels in transformed tobacco plants. Cell 55, 673–681.

8.	Hudson, G. S., Evans, J. R., von Caemmerer, S., Arvidsson, Y. B. C., and Andrews, T. J. (1992) Reduction of ribulose-1, 5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol. 98, 294–302.

9.	Stockhaus, J., Hofer, M., Renger, G., Westerhoff, P., Wydrzynski, T., and Willmitzer, L. (1990) Anti-sense RNA efficiently inhibits formation of the 10 kd polypeptide of photosystem II in transgenic potato plants: analysis of the role of the 10 kd protein. EMBO J. 9, 3013–3021.

10.	Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 12, 8711–8721.

11.	Odell, J. T., Nagy, F., and Chua, N.-H. (1985) Identification of DNA sequences required for the activity of cauliflower mosaic virus 35S promoter. Nature 313, 810–812.

12.	Mazur, B. J. and Chui, C.-F. (1985) Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco. Nucleic Acids Res. 13, 2373–2386.

13.	Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

14.	Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227, 680–685.

15.	Shen, W. and Forde, B. G. (1989) Efficient transformation of Agrobacterium spp. by high voltage electroporation. Nucleic Acids Res. 17, 8385.

16.	Horsch, R. B., Fry, J. E., Hoffmann, N., Eichhloltz, D., Rogers, S. G., and Fraley, R. T. (1985) A simple and general method for transferring genes to plants. Science 227, 1229–1231.

17.	Apel, K. and Kloppstech, K. (1978) The plastid membrane of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light harveting chlorophyll a/b protein. Eur. J. Biochem 85, 581–588.

18.	Vaucheret, H., Kronenberger, J., Lepingle, A., Vilaine, F., Boutin, J.-P., and Caboche, M. (1992) Inhibition of tobacco nitrite reductase activity by expression of antisense RNA. Plant. J. 2, 559–569.

19.	Boutry, M. and Chua, N.-H. (1985) A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J. 4, 2159–2165.

20.	van der Krol, A. R., Mur, L. A., de Lange, P., Mol, J. N. M., and Stuitje, A. R. (1990) Inhibition of flower pimentation by antisense CHS genes, promoter and minimal sequence requirements for antisense effect. Plant Mol. Biol. 14, 457–466.

21.	Adamska, I., Scheel, B., and Kloppstech, K. (1991) Circadian oscillations of nuclear-encoded chloroplast proteins in pea (Pisum sativum). Plant Mol. Biol. 17, 1055–1065.

22.	Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15.

23.	Walker, D. A. (1987). The Use of the Oxygen Electrode and Fluorescence Probes in Simple Measurements of Photosynthesis. Oxygraphics Ltd., Sheffield, UK.

24.	Walker, D. A. (1989) Automated measurement of leaf photosynthetic O₂ evolution as a function of photon flux density. Phil. Trans. R. Soc. Lond. B 323, 313–326.

25.	Quick, W. P., Schurr, U., Scheibe, R., Schulze, E.-D., Rodermel, S. R., Bogorad, L., and Stitt, M. (1991) Decreased ribulose-1,5-bisphosphate carboxylase-oxygenase in transgenic tobacco transformed with “antisense” rbcS. I. Impact on photosynthesis in ambient growth conditions. Planta 183, 542–554.

26.	Stitt, M., Quick, W. P., Schurr, U., Schulze, E.-D., Rodermel, S. R., and Bogorad, L. (1991) Decreased ribulose-1,5-bisphosphate carboxylase-oxygenase in transgenic tobacco transformed with “antisense” rbcS. II. Flux-control coefficients for photosynthesis in varying light, CO₂, and air humidity. Planta 183, 555–566.

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