SpringerProtocols: Abstract: The Plant Oncogenes rolA, B, and C from Agrobacterium rhizogenes: Effects on Morphology, Development, and Hormone Metabolism

The Plant Oncogenes rolA, B, and C from Agrobacterium rhizogenes: Effects on Morphology, Development, and Hormone Metabolism

By: Tony Michael³, Angelo Spena⁴

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The soil pathogen Agrobacterium rhizogenes is the etiological agent of hairy root disease and can incite tumor formation on many dicotyledonous plants (1). The disease is so-called because abundant fine roots that resemble hair develop at the site of infection. A segment of the large Ri plasmid, the T-DNA or transferred DNA, is mobilized from the bacterium into the plant genome, thereby initiating the disease (2–4). The T-DNA may consist of one region (e.g., Ri plasmid 8196) or two separate regions, termed the TL-DNA and the TR-DNA. Axenic growth of transformed roots in liquid culture is typically fast, highly branched, and hormone independent.

Affiliation(s): (3) AFRC Institute of Food Research, Norwich Research Park, Colney, Norwich, UK
(4) Max Planck InstitÃ¼t fÃ¼r ZÃ¼chtungsforschung, KÃ¶ln, Germany

Book Title: Agrobacterium Protocols

Series: Methods in Molecular Biology | Volume: 44 | Pub. Date: Jun-01-1995 | Page Range: 207-222 | DOI: 10.1385/0-89603-302-3:207

Subject: Plant Sciences

References

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