SpringerProtocols: Abstract: 6. Cryopreservation and Freeze-Drying of Fungi

6. Cryopreservation and Freeze-Drying of Fungi

By: Jacqueline A. Kolkowski³, David Smith³

Abstract

Full Text

Download PDF (859K)

Many aspects of mycology today necessitate living cultures. Traditional taxonomy is being supplemented by molecular biological techniques often requiring growing organisms. The need to keep particular strains for confirmation of results, further studies, and as references is well established.

Affiliation(s): (3) Genetic Resources Collection, International Mycological Institute, Egham, Surrey, UK

Book Title: Cryopreservation and Freeze-Drying Protocols

Series: Methods in Molecular Biology | Volume: 38 | Pub. Date: Mar-24-1995 | Page Range: 49-61 | DOI: 10.1385/0-89603-296-5:49

Subject: Cell Biology

References

Download References

1.	Smith, D. and Onions A. H. S. (1983) The Preservation and Maintenance of Living Fungi. Wallingford, UK, CAB Mycological Institute.

2.	Calcott, P. H. (1978) Freezing and Thawing Microbes. Meadowfield Press, Shildon, UK

3.	Franks, F. (1981) Biophysics and biochemistry of low temperatures and freezing, in Esfects of Low Temperature of Biological Membranes (Morris, G. J. and Clarke A., eds.), Academic, London, pp. 3–19.

4.	Smith, D. (1993) Tolerance to freezing and thawing, in Stress Tolerance of Fungi (Jennings, D. H., ed.), Marcel Dekker, New York, pp. 145–171.

5.	Morris, G. J. (1981) Cryopreservution: An Introduction to Cryopreservution in Culture Collections. Culture Centre of Algae and Protozoa, ITE, Cambridge, UK.

6.	Kirsop, B. E. and Doyle, A. (1991) Maintenance of Microorganisms and Cultured Cells: A Manual of Laboratory Methods. Academic, London.

7.	Smith, D. (1988) Culture and maintenance, in Living Resources for Biotechnology: Filumentous Fungi (Hawksworth, D. L. and Kirsop, B. E., eds.), Cambridge University, Cambridge, UK, pp. 75–99.

8.	Hwang, S.-W. (1960) Effects of ultralow temperature on the viability of selected fungus strains. Mycologiu 52, 527–529.

9.	Polge, C., Smith, A. U., and Parkes, S. (1949) Revival of spermatozoa after dehydration at low temperatures. Nature (Lond.) 164, 666.

10.	Morris, G. J., Smith, D., and Coulson, G. E. (1988) A comparative study of the morphology of hyphae during freezing with the viability upon thawing of 20 species of fungi. J. Gen. Microbial. 134, 2897–2906.

11.	Onions, A. H. S. (1983) Preservation of fungi, in The Filumentous Fungi 4, Fungul Technology (Smith, J. E., Berry, D. R, and Kristiansen, B., eds.), Edward Arnold, London, pp. 375–390

12.	Hwang, S.-W. and Howell, A. (1968) Investigation of ultra-low temperature of fungal cultures II. Cryoprotection afforded by glycerol and dimethyl sulfoxide to eight selected fungal cultures. Mycologiu 60, 622–626.

13.	Hwang, S.-W., Kyhx, W. F., and Haynes, W. C. (1976) Investigation of ultra low temperature for fungal cultures III. Viability and growth rate of mycelial cultures cryogenic storage. Mycologiu 68, 377–387.

14.	Ashwood-Smith, M. J. and Warby, C. (1971) Studies on the molecular weight and cryoprotective properties of PVP and Dextran. Cryobiology 8, 453–464.

15.	Smith, D. (1983) Cryoprotectants and the cryopreservation of fungi. Trans. Br. Mycol. Soc. 80, 360–363.

16.	Hwang, S-W. (1966) Long term preservation of fungus cultures with liquid nitrogen refrigeration. Appl. Microbial. 14, 784–788.

17.	Hwang, S.-W. (1968) Investigation of ultra low temperature for fungal cultures I. An evaluation of liquid nitrogen storage for preservation of selected fungal cultures. Mycologia 60, 613–621.

18.	Heckly, R. J. (1978) Preservation of microorganisms. Adv. Appl. Microbial. 24, 1–53.

19.	Raper, K. B. and Alexander, D. F. (1945) Preservation of moulds by the lyophil process. Mycologia 37, 499–525.

20.	Jong, S. C., Levy, A., and Stevenson, R. E. (1984) Life expectancy of freeze-dried fungus cultures stored at 4°C, in Proceedings of the Fourth International Conference on Culture Collections (Kocur, M. and dasilva, E., eds.), World Federation of Culture Collections, London, pp. 125–136.

21.	von Arx, J. A. and Schipper, M. A. A. (1978) The CBS fungus collection. Adv. Appl. Microbial. 24, 215–236.

22.	Smith, D. (1986) The Evaluation and Development of Techniques for the Preservation of Living Fungi. Thesis submitted to London University for the degree of Doctor of Philosophy.

23.	Smith, D. and Kolkowski, J. (1992) Fungi, in Preservation and Maintenance of Cultures Used in Biotechnology and Industry, Butterworth, Stoneham, MA.

24.	Advisory Committee for Dangerous Pathogens (ed.) (1990) Cutegorisution of Pathogens According to Hazard and Categories of Containment. Health and Safety Executive, HMSO, London

25.	The Control of Substances Hazardous to Health (ed.) (1988) Regulations. Health and Safety Commission, London.

26.	Rey, L. R. (1977) Glimpses into the fundamental aspects of freeze drying, in Development in Biological Stundurdisation. International symposium of freeze drying of biological products (Cabasso, V. J., Regamey, R. H., and Krager S., eds.), Basel, Switzerland, pp. 19–27.

27.	Ashwood-Smith, M. J. and Grant, E. (1976) Mutation induction in bacteria by freezedrying. Cryobiology 13, 206–213.

28.	Staffeld, E. E. and Sharp, E. L. (1954) Modified lyophil method for preservation of Pythium species. Phytopathology 44, 213,214.

Comments (Loading...)

Post comment/View all comments