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13. The Xenopus Egg Extract Translation System

By: Glenn M. Matthews², Alan Colman

Abstract

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A full analysis of the posttranslational modifications that a given protein undergoes during transit through the secretory pathway may, in some cases, only be performed by analysis of the natural protein, expressed in its normal tissue.

Affiliation(s): (2) School of Biochemistry, University of Birmingham, UK

Book Title: In Vitro Transcription and Translation Protocols

Series: Methods in Molecular Biology | Volume: 37 | Pub. Date: Jan-19-1995 | Page Range: 199-213 | DOI: 10.1385/0-89603-288-4:199

Subject: Genetics/Genomics

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1.	Matthews, G. M. and Colman, A. (1991) A highly efficient, cell-free translation/translocation system prepared from Xenopus eggs. Nucleic Acids Res. 19, 6405–6412.

2.	Murray, A. W. (1991) Cell cycle extracts, in Methods in Cell Biology, vol. 36 (Kay, B. K. and Peng, H. eds.), Academic, San Diego, pp. 581–605.

3.	Kreig, P. A. and Melton, D. A. (1984) Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs. Nucleic Acids Rex 12, 7057–7070.

4.	Colman, A. (1984) Translation of eukaryotic messenger RNA in Xenopus oocytes, in Transcription and Translation-A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, pp. 271–302.