SpringerProtocols: Abstract: Human Esophageal Culture

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Human Esophageal Culture

By: Carmel Mothersill²

Abstract

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This chapter describes a method for establishing short-term expiant cultures of esophageal mucosa. Adverse effects produced by exposure to radiation or test compounds can be detected as an inhibition of cell outgrowth.

Affiliation(s): (2) Nuclear Energy Board, Dublin, Ireland

Book Title: In Vitro Toxicity Testing Protocols

Series: Methods in Molecular Biology | Volume: 43 | Pub. Date: May-04-1995 | Page Range: 75-79 | DOI: 10.1385/0-89603-282-5:75

Subject: Pharmacology/Toxicology

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1.	Mothersill, C, Cusack, A., MacDonnell, M., Hennessy, T. P., and Seymour, C. B. (1988) Differential response of normal and tumour oesophageal expiant cultures to radiation. Acta Oncologica 27, 275–280

2.	Mothersill, C., Cusack, A., and Seymour, C. B. (1989) Enhanced proliferation of cells from human tissue expiants following irradiation in the presence of environmental carcinogens Radiat. Environ Biophys. 28, 203–212.