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Stages in ELISA

Abstract

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This chapter gives general information on common practical features of the ELISA, featuring the main elements of:

1.	The adsorption of antigen or antibody to the plastic solid-phase.
2.	The addition of the test sample and subsequent reagents.
3.	The incubation of reactants.
4.	The separation of bound and free reactants by washing.
5.	The addition of enzyme-labeled reagent.
6.	The addition of enzyme detection system (color development).
7.	The visual or spectrophotometric reading of the assay.

Book Title: ELISA: Theory and Practice

Series: Methods in Molecular Biology | Volume: 42 | Pub. Date: May-03-1995 | Page Range: 63-97 | DOI: 10.1385/0-89603-279-5:63

Subject: Immunology

References

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