SpringerProtocols: Abstract: Evaluation of Antibody Clonality

Evaluation of Antibody Clonality

By: L. Scott Rodkey²

Abstract

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The seminal development shaping our current understanding of the immune system was the formulation of the concept of clonal selection by Sir Macfarlane Burnet (1). This idea revolutionized the approach to formulation of experimental design in immunology. At the heart of clonal selection was the idea that antibody specificity preexisted before antigen arrival and that the antigen triggered the system by selecting only cells preprogrammed to make antibody molecules with significant affinity for that particular antigen. The former instructional theories of antibody formation clearly were rendered obsolete by this concept. Many scholars attribute the subsequent formal proof of clonal selection to Raff et al. (2).

Affiliation(s): (2) Department of Pathology, University of Texas-Houston Medical School, Houston, TX

Book Title: Antibody Engineering Protocols

Series: Methods in Molecular Biology | Volume: 51 | Pub. Date: Jul-13-1995 | Page Range: 151-163 | DOI: 10.1385/0-89603-275-2:151

Subject: Immunology

References

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1.	Burnet, F. M. (1959) The clonal selection theory of acquired immunity. Cambridge University Press: London, England.

2.	Raff, M., Feldmann, M., and de Petris, S. (1973) Monospecificity of bone marrow derived lymphocytes. J. Exp. Med. 137, 1024–1030.

3.	Jerne, N. K. and Nordin, A. A. (1963) Plaque formation in agar by single antibody-producing cells. Science 140, 405.

4.	Dresser, D. W. and Wortis, H. H. (1965) Use of an antiglobulin serum to detect cells producing antibody with low haemolytic efficiency. Nature 208, 859–861.

5.	Vesterberg, O. (1969) Synthesis and isoelectric fractionation of carrier ampholytes. Acta. Chem. Stand. 23, 2653–2666.

6.	Awdeh, Z. L., Williamson, A. R., and Askonas, B. A. (1968) Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature 219, 66,67.

7.	Dale, G. and Latner, A. L. (1968) Isoelectric focusing of serum proteins in acrylamide gels followed by electrophoresis. Clin. Chim. Acta 24, 61–68.

8.	Fawcett, J. S. (1968) Isoelectric fractionation of proteins on polyacrylamide gels. FEBS Lett. 1, 81,82.

9.	Leaback, D. H. and Rutter, A. C. (1968) Polyacrylamide-isoelectric-focusing A new technique for the electrophoresis of proteins. Biochem. Biophys. Res. Commun. 32, 447–453.

10.	Riley, R. F. and Coleman, M. K. (1968) Isoelectric fractionation of proteins on a microscale in polyacrylamide and agarose matrices. J. Lab. Clin. Med. 72, 714–720.

11.	Wrigley, C. W. (1968) Analytical fractionation of plant and animal proteins by gel electrofocusing. J. Chromatogr. 36, 362–365.

12.	Awdeh, Z. L., Williamson, A. R., and Askonas, B. A. (1970) One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins. Biochem. J. 116, 241–248.

13.	Askonas, B. A., Williamson, A. R., and Wright, B. E. G. (1970) Selection of a single antibody-forming cell clone and its propagation in syngeneic mice. Proc. Natl. Acad. Sci. USA 67, 1398–1403.

14.	Keck, K., Grossberg, A. L., and Pressman, D. (1973) Specific characterization of isoelectrofocused immunoglobulins in polyacrylamide gel by reaction with ¹²⁵I-labeled protein antigens or antibodies. Eur. J. Immunol. 3, 99–102.

15.	Braun, D. G., Hild, K., and Ziegler, A. (1979) Isoelectric focusing of immunoglobulins, in Immunological Methods. (Lefkovits, I. and Pernis, B., eds.), Academic, New York, pp. 107–121.

16.	Schalch, W. and Braun, D. (1979) Isolation of monoclonal antibody by preparative isoelectric focusing in horizontal layers of Sephadex G-75, in Immunological Methods. (Lefkovits, I. and Pernis, B., eds.), Academic, New York, pp. 123–130.

17.	Schalch, W., Wright, J. K., Rodkey, L. S., and Braun, D. G. (1979) Distinct functions of monoclonal IgG antibody depend on antigen site specificities. J. Exp. Med. 149, 923–937.

18.	Brown, J. C. and Rodkey, L. S. (1979) Autoregulation of an antibody response via network-induced auto-anti-idiotype. J. Exp. Med. 150, 67–85.

19.	Binion, S. B., Rodkey L. S., Egen, N. B., and Bier, M. (1982) Rapid purification of antibodies to single band purity using recycling isoelectric focusing. Electrophoresis 3, 284–288.

20.	Binion, S. B. and Rodkey, L. S. (1982) Isoelectric focusing studies of antibody clonotype patterns in outbred rabbits during prolonged immunization. Electrophoresis 3, 289–293.

21.	Insel, R. A., Kittelberger, A., and Anderson, P. (1985) Isoelectric focusing of human antibody to the Haemophilus influenza b capsular polysaccharide: restricted and Identical spectrotypes in adults. J. Immunol. 135, 2810–2816.

22.	D’ Amelio, R., Del Guidice, G., De Rosa, F., Brighouse, G., Teggi, T., and Lambert, P. H. (1986) Spectrotypic analysis of humoral response in human hydatidosis. Clin. Exp. Immunol. 76, 117–120.

23.	Le Moli, S, Matricardi, P. M., Quinti, I., Stroffolini, T., and D’ Amelio, R. (1991) Clonotypic analysis of human antibodies specific for Neisseria meningitidis polysaccharides A and C in adults. Clin. Exp. Immunol. 83, 460–465.

24.	Roos, R. P., Nalefski, E. A., Nitayaphan, S., Variakijis, R., and Singh, K. K. (1987) An isoelectric focusing overlay study of the humoral immune response in Theiler’s virus demyelinating disease. J. Neuroimmunol. 13, 305–314.

25.	Stott, D. I., Hassman, R., Neilson, L., and McGregor, A. M. (1988) Analysis of the spectrotypes of autoantibodies against thyroglobulin in two rat models of autoimmune thyroiditis. Clin. Exp. Immunol. 73, 269–275.

26.	Muryoi, T., Sasaki, T., Hatakeyama, A., Shibata, S., Suzuki, M., Seino, J., and Yoshinaga, K. (1990) Clonotypes of anti-DNA antibodies expressing specific idiotypes in immune complexes of patients with active Lupus nephritis. J. Immunol. 144, 3856–3861.

27.	Stott, D. I. (1992) Spectrotypes of anti-DNA antibodies show that anti-DNA-secreting B-cell clones of SLE patients are restricted in number, stable and long lived. Autoimmunity 12, 249–258.

28.	D’ Amelio, R., Biselli, R., Nisini, R., Matricardi, P. M., Aiuti, A., Mezzaroma, I., Pinter, E., Pontesilli, O., and Aiuti, F. (1992) Spectrotype of anti-gp120 antibodies remains stable during the course of HIV disease. J. Acquired Immune Deficiency Syndromes 5, 930–935.

29.	Knisley, K. A. and Rodkey, L. S. (1986) Affinity Immunoblotting Hugh resolution isoelectric focusing analysis of antibody clonotype distribution. J. Immunol. Methods 95, 79–87.

30.	Knisley, K. A. and Rodkey, L. S. (1988) Isoelectric focusing analysis of antibody clonotype changes occurring during immune responses using immobilized pH gradients. Electrophoresis 9, 183–186.

31.	Mehrazar, K., Gilman-Sachs, A., Knisley, K. A., Rodkey, L. S., and Kim, Y. B. (1993) Comparison of the immune response to ARS-BGG in germfree or conventional piglets. Dev. Compar. Immunol. 17, 459–464.

32.	Seferian, P. G., Rodkey, L. S., and Adler, F. L. (1987) Selective survival and expression of B-lymphocyte memory cells during long-term serial transplantation. Cell. Immunol. 110, 226–232.

33.	Adler, F. L., Adler, L. T., Seferian, P. G., and Rodkey, L. S. (1989) Adoptive immunity transferred by naive donor cells immunized in vitro. Bone Marrow Transplant 4, 663–668.

34.	Labadie, J., van Tol, M. J. D., Dijkstra, N. H., van der Kaaden, M., Jol-van der Zijde, C. M., de Lange, G. G., Zwaan, F. E., and Vossen, J. M. (1992) Transfer of specific immunity from donor to recipient of an allogeneic bone marrow graft: evidence for donor origin of the antibody producing cells. B. J. Hematol. 82, 437–444.

35.	Hamilton, R. G., Roebber, M., Reimer, C. B., and Rodkey, L. S. (1987) Isoelectric focusing-affinity immunoblot analysis of mouse monoclonal antibodies to the four human IgG subclasses. Electrophoresis 8, 127–134.

36.	Hamilton, R. G., Roebber, M., Reimer, C. B., and Rodkey, L. S. (1987) Quality control of murine monoclonal antibodies using isoelectric focusing affinity immunoblot analysis. Hybridoma 6, 205–217.

37.	Rieben, R., Fraunfelder, A., and Nydegger, U. E. (1992) Naturally occurring ABO antibodies: long-term stable, individually distinct anti-A IgG spectrotypes. Eur. J. Immunol. 22, 2129–2133.

38.	Rieben, R., Frauenfelder, A., and Nydegger, U. E. (1994) Clonality of naturally occurring antibodies to histo-blood group substances A and B from human serum and breast milk revealed by spectrotype analysis. Blood, in press.

39.	Brown, R. M. and Krolick, K. A. (1988) Clonotypic analysis of the antibody response to the acetylcholine receptor in experimental autoimmune myasthenia gravis. J. Neuroimmunol. 19, 205–222.

40.	Yeh, T. M. and Krolick, K. A. (1989) Clonotypic analysis of anti-acetylcholine receptor antibodies produced against native and denatured antigen. J. Neuroimmunol. 24, 133–142.

41.	Thompson, P. A. and Krolick, K. A. (1992) Acetylcholine receptor-reactive antibodies in experimental autoimmune myasthenia gravis differing in disease-causing potential: subsetting of serum antibodies by preparative isoelectric focusing. Clin. Immunol. Immunopathol. 62, 199–209.

42.	Mohan, S., Barohn, R. J., and Krolick, K. A. (1992) Unexpected cross-reactivity between myosin and a main immunogenic region (MIR) of the acetylcholine receptor by antisera obtained from myasthenia gravis patients. Clin. Immunol. Immunopathol. 64, 218–226.

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