SpringerProtocols: Abstract: 4. Insect Cell Culture in Serum- Free Media

4. Insect Cell Culture in Serum- Free Media

By: Stefan A Weiss³, Glenn P. Godwin⁴, Stephen F Gorfien⁴, William G. Whitford⁴

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Historically, insect cell culture was considered a method for the production of viral pesticides (1, 2). To support basic research and to explore potential replication of entomopathogenic viruses in vitro, there have been over 400 cell lines established to date from various insect species (3). Without exception, the cell lines described in the literature and currently in use have been isolated and established from primary cultures using serum-supplemented insect cell-culture media (4, 5; see also Chapter 3 in this volume).

Affiliation(s): (3) Biosys, Palo Alto, CA
(4) G IBCO/BRL Life Technologies Inc., Cell Culture Research and Development, Grand Island, NY

Book Title: Baculovirus Expression Protocols

Series: Methods in Molecular Biology | Volume: 39 | Pub. Date: Feb-20-1995 | Page Range: 79-95 | DOI: 10.1385/0-89603-272-8:79

Subject: Microbiology

References

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2.	Weiss, S. A., Smith, G. C., Kalter, S. S., Vaughn, J. L., and Dougherty, E. (1981) Improved replication of Autographu californica NPV in roller bottles: Characterization of the progeny virus. Intervirology 15, 213–222.

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13.	Weiss, S. A., Gortien, S., Fike, R., DiSorbo, D., and Jayme, D. (1990) Large-scale production of proteins using serum-free insect cell culture, in Biotechnology: The Science and the Business. Proceedings for Ninth Australian Biotechnology Conference, Queensland, pp. 220–231.

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16.	Weiss, S. A., DiSorbo, D. M,, Whitford, W. G., and Godwin, G. (1991) Improved production of recombinant proteins in high density insect cell culture. In Vitro Cell. Devel. Biol. 27#3, 42A.

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21.	Weiss, S. A., Peplow, D., Smith, G. C., and Goodwin, R. H. (1984) Replication of Heliothis zeu baculovirus in insect cells grown in serum-free media (SFM). In Vitro Cell. and Devel. Biol. 20, 271.

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24.	Miltenburger, H. G. (1983) Investigation on cultivation of insect cell lines in serum-free media. European Conference on Hormonally Defined Media 1, 31–43.

25.	Weiss, S. A., Godwin, G. P., Gorfien, S. F., and Whitford, W. G. (1992) Viral Pesticides: In vitro process development. Proceedings for 10th Annual Australian Biotechnology Association Conference (Melbourne).

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