SpringerProtocols: Abstract: Nonradioactive Labeling of Polymerase Chain Reaction Products

Nonradioactive Labeling of Polymerase Chain Reaction Products

By: Udo Reischl², RÃ¼diger RÃ¼ger², Christoph Kessler²

Abstract

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Polymerase chain reaction (PCR) was originally introduced to amplify in vitro particular DNA sequences by the application of temperature cycles (1). In a modification, RNA molecules also may serve as templates by an additional reverse transcription step converting RNA in complementary DNA sequences (2).

Affiliation(s): (2) Department of Molecular Genetics, Boehringer Mannheim GmbH, Penzberg/Obb., Germany

Book Title: PCR Protocols: Current Methods and Applications

Series: Methods in Molecular Biology | Volume: 15 | Pub. Date: Feb-03-1993 | Page Range: 51-62 | DOI: 10.1385/0-89603-244-2:51

Subject: Genetics/Genomics

References

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