SpringerProtocols: Abstract: Determination of Loss of Heterozygosity Using Polymerase Chain Reaction

Determination of Loss of Heterozygosity Using Polymerase Chain Reaction

By: Stephen J. Meltzer²

Abstract

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Loss of heterozygosity (LOH) is being detected with increasing frequency in a wide variety of human tumors (1-10). Frequent LOH at a given chromosomal locus implies the existence of a tumor suppressor gene that is important in the pathogenesis of the particular cancer under study. LOH is believed to represent inactivation of one allele of a tumor suppressor gene by chromosomal or subchromosomal deletion. LOH studies have led to the isolation of new tumor suppressor genes (11-13). They have also pinpointed known tumor suppressor genes that may be significant in specific types of cancer (14,15).

Affiliation(s): (2) University of Maryland Hospital, Baltimore, MD

Book Title: PCR Protocols: Current Methods and Applications

Series: Methods in Molecular Biology | Volume: 15 | Pub. Date: Feb-03-1993 | Page Range: 129-136 | DOI: 10.1385/0-89603-244-2:129

Subject: Genetics/Genomics

References

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