SpringerProtocols: Abstract: 252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins

252-Californium Plasma Desorption Time-of-Flight Mass Spectrometry of Peptides and Proteins

By: Peter Roepstorff²

Abstract

Full Text

Download PDF (390K)

Plasma desorption mass spectrometry (PDMS) (1) is a method for the mol-wt determination of peptides and small proteins. The upper mass limit is, in optimal cases, approx 30 kDa, with a precision of about 0.1%. This precision far exceeds that of classical biochemical methods, such as SDS-gel electrophoresis or gel permeation chromatography. The molecular weights determined by PDMS depend only on the atomic composition of the molecule and not, as in other methods, on extraneous properties, such as hydrophobicity or shape.

Affiliation(s): (2) Department of Molecular Biology, Odense University, Odense, Denmark

Book Title: Spectroscopic Methods and Analyses: NMR, Mass Spectrometry, and Metalloprotein Techniques

Series: Methods in Molecular Biology | Volume: 17 | Pub. Date: May-23-1993 | Page Range: 229-235 | DOI: 10.1385/0-89603-215-9:229

Subject: Cell Biology

References

Download References

1.	Thorgerson, D. F, Skowronski, R P., and Macfarlane D. F. (1974) New approach to the mass spectrometry of non-volatile compounds. Biochem Biophys. Res Comm. 60, 616–621.

2.	Roepstorff, P (1989) Plasma desorption mass spectrometry of peptides and proteins Acc Chem. Res. 22, 421–427.

3.	Mann, M, Nielsen, H R, and Roepstorff, P. (1990) Practical aspects of calibration and effect of non-protein compounds on spectrum quality in protein analysis by PDMS, in Ion Formation from Organic Solids (Hedin, A., Sundqvist, B U R, and Benninghoven, A, eds.), Wiley, Chichester, UK, pp. 47–54.

4.	Jonsson, G. P., Hedin, A B., Håkansson, P. L, Sundqvist, B U R., Säve, B. G, Nielsen, P. F, Roepstorff, P., Johansson, K. E, Kamensky, I, and Lindberg, M S L. (1986) Plasma desorption mass spectrometry of peptides and proteins adsorbed on nitrocellulose. Anal. Chem. 58, 1084–1087.

5.	Nielsen, P F., Klarskov, K, Højrup, P., and Roepstorff, P (1988) Optimization of sample preparation for plasma desorption mass spectrometry of peptides and proteins using a nitrocellulose matrix Biomed Environ Mass Spectrom 17, 355–362.

6.	Chait, B T., Chaudhary, T., and Field, F. H. (1987) Mass spectrometric characterization of microscale enzyme catalyzed and chemical reactions on surface bound peptides and proteins, in Methods in Protein Sequence Analysis (Walsh, K. A, ed.), Humana, Clifton, NJ, pp. 483–492.

7.	Nielsen, P. F. and Roepstorff, P (1988) Suppression effects in peptide mapping by plasma desorption mass spectrometry. Biomed Environ Mass Spectrom 18, 131–137.

8.	Nielsen, P. F., Roepstorff, P., Clausen, I. B., Jensen, E G, Jonassen, I, Svendsen, A, Balschmidt, P, and Hansen, F. B. (1989) Plasma desorption mass spectrometry, an analytical tool in protein engineering: characterisation of modified insulins. Protein Eng. 2, 449–457.

9.	Klarskov, K, Breddam, K., and Roepstorff, P (1989) C-Terminal sequence determination of peptides degraded with carboxypeptidases of different specificities and analysed by²⁵²Cf plasma desorption mass spectrometry Anal Biochem 180, 28–37.

Comments (Loading...)

Post comment/View all comments