SpringerProtocols: Abstract: Irradiation and Fusion Gene Transfer

Irradiation and Fusion Gene Transfer

By: Peter N. Goodfellow²

Abstract

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The fusion of human and rodent somatic cells is usually followed by the spontaneous loss of human chromosomes from the hybrid cells (1). The correlation between the presence of a human chromosome retained in the hybrid cell and the presence of a human phenotypic trait is the basis of human gene mapping by somatic cell genetics (2). This method for constructing physical maps has been central to human genetics and has resulted in the chromosomal assignment of several hundred genes (see ref. 3). Unfortunately, subchromosomal gene localization by this approach is limited by the paucity of suitable translocation and deletion chromosomes.

Affiliation(s): (2) Imperial Cancer Research Fund, London, UK

Book Title: Gene Transfer and Expression Protocols

Series: Methods in Molecular Biology | Volume: 7 | Pub. Date: Apr-22-1991 | Page Range: 53-61 | DOI: 10.1385/0-89603-178-0:53

Subject: Genetics/Genomics

References

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