SpringerProtocols: Abstract: Calcium Phosphate Mediated Gene Transfer into Established Cell Lines

Calcium Phosphate Mediated Gene Transfer into Established Cell Lines

By: Hiroto Okayama², Claudia Chen²

Abstract

Full Text

Download PDF (458K)

DNA transfection is one of the most important techniques in molecular genetics. It is this technique that has made possible the dissection of complex eukaryotic genes and the characterization of the function of their components (1–7) as well as the isolation of particular genes on the basis of their expression in cultured cells (8–11).

Affiliation(s): (2) Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD, Osaka, Japan

Book Title: Gene Transfer and Expression Protocols

Series: Methods in Molecular Biology | Volume: 7 | Pub. Date: Apr-22-1991 | Page Range: 15-21 | DOI: 10.1385/0-89603-178-0:15

Subject: Genetics/Genomics

References

Download References

1.	Banerji, J., Olson, L., and Schaffner, W. (1983) A lymohocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell 33, 727–740.

2.	Benoist, C. and Chambon, P. (1981) In vivo sequence requirements of the SV40 early region. Nature 290, 304–309.

3.	Fromm, M. and Berg, P. (1982) Deletion mapping of DNA regions required for SV40 early promoter function in uivo. J Mol. Appl. Genet. 1, 457–481.

4.	Ghosh, P. K, Lebowitz, P., Frisque, R. J., and Gluzman, Y. (1981) Identification of a promoter component involved in positioning the 5’ termini of simian virus 40 early mRNAs. Proc. Natl. Acad. Sci. USA 78, 100–104.

5.	Queen, C. and Baltimore, D. (1983) Immunoglobulin gene transcription is activated by downstream sequence elements.cell 33,741–748.

6.	Schechter, A. L., Stem, D. F., Vaidyanathan, L., Decker, S. J., Drebin, J. A., Greene, M. I., and Weinberg, R. A. (1984) The nue oncogene: An erb-B-related gene encoding a 185,000-M, tumour antigen. Nature 321, 513–616.

7.	Shih, C. and Weinberg, R. A. (1982) Isolation of a transforming sequence from a human bladder carcinoma cell line.Cell 29, 161–169.

8.	Shimizu, K., Coldfarb, M., Perucho, M., and Wigler, M. (1983) Isolation and preliminary characterization of the transforming gene of a human neuroblastoma cell line. Proc. Natl. Acad. Sci. USA 80, 383–387.

9.	Westerveld, A., Hoeijimakers, H. J., van Duin, M., deWit, J,, Odijk, H., Pastink, A. R. D. Wood, R. D., and Bootsma, D. (1984) Molecular cloning of a human DNA repair gene. Nature 310, 425–429.

10.	Jolly, D.J., Esty, A. C., Bernard, H.U., and Friedman, T. (1982) Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyl transferase. Proc. Natl.Acad. Sci. USA 79, 5038–5041.

11.	Noda, M., Kitayama, H., Matsuzaki, T., Sugimoto, Y., Okayama, H., Bassin, R. H., and Ikawa, Y. (1989) Detection of genes with a potential for suppressing the transformed phenotype associated with activated ras genes. Proc. Natl. Acad. Sci. USA 86, 162–166.

12.	Graham, F. L. and van der Eb, A. J. (1973) A new technique for the assay of infectivity of human adenovirus 5.Virology 52, 456–467.

13.	Parker, B. A. and Strak, G. R. (1919) Regulation of simian virus 40 transcription: Sensitive analysis of the RNA species present early in infections by virus or viral DNA. J Viral. 31, 360–369.

14.	Lewis, W. H., Strinivasan, P. R., Stokoe, N., and Siminovitch, L. (1980) Parameters governing the transfer of the genes for the thymidine kinase and dihydrofolate reductase into mouse cells using metephase chromosomes or DNA. Somatic Cell Genet. 6, 333–348.

15.	Faber, F. E. and Eberle, R. (1976) Effects of cytochalasin and alkaloid drugs on the biological expression of herpes simplex virus type 2 DNA. Exp. Cell Res. 103, 15–22.

16.	Luthman, H. and Magnusson, G. (1983) High efficiency polyoma DNA transfection of chloroquine treated cells. Nuctic Acids Res. 11, 1295–1307.

17.	Chen, C. and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745–2752.

18.	Chen, C. A. and Okayama, H. (1988) Calcium phosphate-mediated gene transfer: A highly efficient transfection system for stably transforming cells with plasmid DNA. Bwotechniques 6, 632–638.

19.	Mulligan, R. C. and Berg, P. (1981) Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Null. Acad. Sci. USA 78, 2072–2076.

20.	Kaster, K R, Burgett, S. G., Nagaraja, R, and Ingolia, T. D. (1983) Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusion and by DNA sequencing. Nuckk Acids Res. 11, 6895–6911.

Comments (Loading...)

Post comment/View all comments