SpringerProtocols: Abstract: Manipulation of Adenovirus Vectors

Manipulation of Adenovirus Vectors

By: Frank L. Graham², Ludvik Prevec³

Abstract

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Adenoviruses have been isolated from a large number of different species (mammalian and fowl) and over 100 different serotypes have been reported, some 43 of them human. The human adenoviruses, particularly types 2, 5, and 12, have been the most extensively characterized, and these viruses have served as valuable tools in the study of the molecular biology of DNA replication, transcription, RNA processing, and protein synthesis in mammalian cells. Seeref. 1 for a general review.

Affiliation(s): (2) Departments of Biology and Patholo, McMaster University, Hamilton, Ontario, Canada
(3) McMaster University, Hamilton, Ontario, Canada

Book Title: Gene Transfer and Expression Protocols

Series: Methods in Molecular Biology | Volume: 7 | Pub. Date: Apr-22-1991 | Page Range: 109-128 | DOI: 10.1385/0-89603-178-0:109

Subject: Genetics/Genomics

References

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1.	Ginsberg, H. S. (1984) The Adenoviruses (Plenum, New York).

2.	Berkner, K. L. (1988) Development of adenovirus vectors for expression of heterolo gous genes. BioTechnigues 6, 616–629.

3.	Jones, N. and Shenk, T. (1979) Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell 16, 683–689.

4.	Graham, F. L., Smiley, J., Russell, W. C., and Nairn, R. (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen. Virol. 36, 59–72.

5.	Ghosh-Choudhury, G., Haj-Ahmad, Y., and Graham, F. L. (1987) Protein IX, a minor component of the human adenovirus capsid, is essential for the packaging of full length genomes. EMBOJ 6, 1733–1739.

6.	Berkner, K L. and Sharp, P. A. (1983) Generation of adenovirus by transfection of plasmids. Nucleic Acids Res. 11, 6003–6020.

7.	Haj-Ahmad, Y. and Graham, F. L. (1986) Development of a helper independent human adenovirus vector and its use in the transfer of the Herpes Simplex Virus thy-midine kinase gene. J Krol. 57, 267–274.

8.	Johnson, D. C., GhoshChoudhury, G., SmiIey, J. R., Fallis, L., and Graham, F. L. (1988) Abundant expression of Herpes Simplex Virus glycoprotein gB using an adenovirus vector. Virology 164, 1–14.

9.	Schneider, M., Graham, F. L., and Prevec, L. (1989) Expression of the gIycoprotein of VSV by infectious adenovirus vectors. J. Gen, Virol. 70, 417–427.

10.	Davis, A. R., Kostek, B., Mason, B. B., Hsiao, C. L., Morin, J., Dheer, S. EC, and Hung, P. P. (1985) Expression of hepatitis B virus surface antigen with a recombinant adenovirus. Proc. Natl. Acad. Sci. USA 82, 7560–7564.

11.	Spessot, R., Inchley, K, Hupel, T. M., and Bacchetti, S. (1989) Cloning of the herpes simplex virus ICP4 gene in an adenovirus vector: Effects on adenovirus gene expression and replication. Virolology 168, 378–387.

12.	Ruben, M., Bacchetti, S., and Graham, F. L. (1983) Covalently closed circles of human adenovirus type 5 DNA. Nature 3O1, 172–174.

13.	Graham, F. L. (1984) Covalently closed circles of human adenovirus DNA are infectious. EMBOJ 3 2917–2922.

14.	Ghosh-Choudhury, G., Haj-Ahmad, Y., Brinkley, P., Rudy, J., and Graham, F. L. (1986)Human adenovirus cloning vectors based on infectious bacterial plasmids. Gene 50, 161–171.

15.	McGroty, J., Bautista, D., and Graham, F. L. (1988) A simple technique for the rescue of early region I mutations into infectious human adenovirus type 5. Virology 168, 614–617.

16.	Graham, F. L. (1981) Growth of 293 cells in suspension culture. J Gen. Kirol. 68, 937–940.

17.	Sharp, P. A., Moore, C., and Haverty, J. L. (1976) The infectivity of adenovirus 5 DNA-protein complex. Virology 75, 442–456.

18.	Chinnadurai, G., Chinnadurai, S., and Green, M. (1978) Enhanced infectivity of adenovirus type 2 DNA and a DNA-protein complex. J. Virol 26, 195–199.

19.	Graham, F. L. and van der Eb, A.J. (1973) A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52, 456–467.

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