SpringerProtocols: Abstract: Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines

Establishment of Mouse Epithelial Lung Cell Strains and Cell Lines

By: Garry J. Smith³

Abstract

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Mesenchymal cells have been cultured successfully for many years owing to the ease of their culturability. With the advent of more complex growth media, and some changes to methods, it has become possible to culture epithelial cell types from a wide variety of rodent and human tissue sources successfully (1,2,3). We have used medium CMRL1066 with serum to establish and cultivate lung cell lines that are related to the type II pneumocyte of lung alveolus (4–7). Other laboratories have successfully cultured different types of lung cells, including tracheal and bronchial cell types (1,2,3,8).

Affiliation(s): (3) School of Pathology, University of New South Wales, Kensington, Australia

Book Title: Animal Cell Culture

Series: Methods in Molecular Biology | Volume: 5 | Pub. Date: Jan-30-1990 | Page Range: 99-111 | DOI: 10.1385/0-89603-150-0:99

Subject: Cell Biology

References

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1.	Franks, L. M. and Wigley, C. B. (eds.) (1979) Neoplastic Transformation in Differentiated Epithelial Cell Systems in Vitro (Academic Press, London).

2.	Harris, C. C. (1987) Human tissues and cells in carcinogenesis research. Cancer Res. 47, 1–10.

3.	Smith, G. J. and Stewart, B. W. (eds.) (1982) In Vitro Epithelial Cell Differentiation and Neoplasia (The Australian Cancer Soc., Sydney).

4.	Smith, G. J., Le Mesurier, S. M., DeMontfort, M. L., and Lykke, A. W. J. (1984) Establishment of epithelial cell strains from normal adult mouse lung resembling a urethane-induced lung adenoma cell strain and a metastasing mouse lung carcinoma cell line. Cell Biol. International Reports 8, 161–169.

5.	Smith G. J., Le Mesurier, S. M., DeMontfort, M. L., and Lykke, A. W. J. (1984) Development and characterization of type II pneumocyte related cell lines from normal mouse lung. Pathology 16, 401–405.

6.	Smith, G. J. and Lykke, A. W. J. (1985) Characterization of a neoplastic epithelial cell strain derived by dexamethasone treatment of cultured normal mouse type II pneumocytes. J. of Pathology 147, 165–172.

7.	Smith, G. J., Bennett, F. A., Steele, J. G., and Bentel, J. M. (1986) Onset of neoplastic phenotype in an epithelial cell strain from adult BALB/c mouse lung alveolus. J. Natl. Cancer Inst. 76, 73–79.

8.	Nettesheim, P., Terzaghi, M., and Klein-Szanto, A. J. P. (1982) Development and progression of neoplastic disease. Morphologic and cell culture studies with airway epithelium, in Mechanisms of Chemical Carcinogenesis (Harris, C. C., ed.), Liss, New York, pp. 473–489.

9.	Ward, J. M., Singh, G., Katyal, S. L., Anderson, L. M., and Kovatch, R. M. (1985) Immunocytochemical localization of the surfactant apoprotein and clara cell antigen in chemically induced and naturally occurring pulmonary neoplasms of mice. Amer. J.Pathol. 118, 493–499.

10.	Smith, G. J., Kumar, R. K., Hristoforidis, C. P., and Lykke, A. W. J. (1985) Expression of a type II pneumocyte-specific antigen by a cell strain from normal adult mouse lung. Cell Biol. International Reports 9, 1115–1122.

11.	Paul, J. (1975) Cell and Tissue Culture, 5th Ed. (Churchill-Livingstone, London).

12.	Kuchler, R. J. (1977) Biochemical Methods in Cell Culture and Virology. (Dowden, Hutchinson and Ross, Stroudsburg, Pennsylvania).

13.	Kao, W. W.-Y. and Prockop, D. J. (1977) Proline analogue removes fibroblasts from cultured mixed cell populations. Nature 266, 63–64.

14.	Edwards, A. M. and Lucas, C. M. (1982) Gamma-glutamyltranspeptidase as a pre-neoplastic marker in hepato carcinogenesis: Expression in hepatocytes isolated from normal and carcinogen-treated rats, in In Vitro Epithelial Cell Differentiation and Neoplasia (Smith, G. J. and Stewart, B. W., eds.) The Australian Cancer Soc., Sydney.

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