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2. In Vitro Propagation of Jojoba
Jojoba (Simmondsia chinensis (Link) Schn.) is a nontraditional crop in arid and semi-arid areas. Vegetative propagation can be achieved by layering, grafting, or rooting semi-hardwood cuttings, but the highest number of possible propagules is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture initiation, single-node explants are cultivated on Murashige and Skoog medium (MS) supplemented with Gamborg’s vitamins (B5), 11.1 μM BA (N6-benzyl-adenine), 0.5 μM IBA (indole-3-butyric acid), and 1.4 μM GA3 (gibberellic acid). Internodal and apical cuttings proliferate on MS medium containing B5 vitamins and 4.4 μM BA. Rooting is achieved on MS medium (half strength mineral salt) amended with B5 vitamins and 14.7 μM IBA during 7 days and transferred to develop in auxin-free rooting medium. Plantlets are acclimatized using a graduated humidity regime on soil: peat: perlite (5:1:1) substrate. This micropagation protocol produces large numbers of uniform plants from selected genotypes of jojoba.
Affiliation(s): (1) Plant Tissue Culture Laboratory (CULTEV), Department of Basic Sciences, National University of Luján, Buenos Aires, Argentina
(2) Department of Basic Sciences, Plant Tissue Culture Laboratory (CULTEV), National University of Luján, Buenos Aires, Argentina
Series: Methods in Molecular Biology  |  Volume: 994  |  Pub. Date: Oct-01-2012  |  Page Range: 19-31  |  DOI: 10.1007/978-1-62703-074-8_2
Subject:  Plant Sciences
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