Contents of this article

Useful Tools
1. Rapid LC–MS/MS Profiling of Protein Amino Acids and Metabolically Related Compounds for Large-Scale Assessment of Metabolic Phenotypes
Amino acids extracted from a biological matrix can be resolved and measured using a 6-min per sample method through high-performance liquid chromatography with a short C18 column and rapid gradient using the ion-pairing reagent perfluoroheptanoic acid. LC-tandem mass spectrometry with multiple reaction monitoring (MRM) transitions selective for each compound allows simultaneous quantification of the 20 proteinogenic amino acids and 5 metabolically related compounds. Distinct MRM transitions were also established for selective detection of the isomers leucine/isoleucine and threonine/homoserine.
Affiliation(s): (1) Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA
(2) Department of Biochemistry and Molecular Biology, and Department of Chemistry, Michigan State University, East Lansing, MI, USA
(3) Departments of Biochemistry and Molecular Biology and Department of Plant Biology, Michigan State University, East Lansing, MI, USA
Series: Methods in Molecular Biology  |  Volume: 828  |  Pub. Date: Dec-02-2011  |  Page Range: 1-11  |  DOI: 10.1007/978-1-61779-445-2_1
Subject:  Protein Science
Comments (Loading...)