SpringerProtocols: Abstract: Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions

6. Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions

By: Frédéric Minner¹, Françoise Herphelin¹, Yves Poumay¹

Abstract

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This chapter deals with protocols to set up human keratinocyte cultures in serum-free conditions and lead them to autocrine autonomously growing conditions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by comparison with adequate housekeeping genes.

Affiliation(s): (1) Cell and Tissue Laboratory, URPHYM, University of Namur (FUNDP), Namur, Belgium

Book Title: Epidermal Cells: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 585 | Pub. Date: Oct-07-2009 | Page Range: 71-82 | DOI: 10.1007/978-1-60761-380-0_6

Subject: Cell Biology

Key Words: Keratinocytes - Autocrine culture - Cell density - Epidermal differentiation markers - Quantitative RT-PCR

References

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