SpringerProtocols: Abstract: Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics

1. Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics

By: Michael A. Kiebish¹, Xianlin Han², Thomas N. Seyfried³

Abstract

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Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.

Affiliation(s): (1) Biology Department, Boston College, Boston, MA, USA
(2) Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, USA
(3) Department of Biology, Boston College, Chestnut Hill, MA, USA

Book Title: Lipidomics: Volume 1: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 579 | Pub. Date: Oct-02-2009 | Page Range: 3-18 | DOI: 10.1007/978-1-60761-322-0_1

Subject: Cell Biology

Key Words: Nonsynaptic - Synaptic - Mitochondria - Brain - Lipidome

References

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