SpringerProtocols: Abstract: Liquid Chromatography?Mass Spectrometry-Based Quantitative Proteomics

11. Liquid Chromatography–Mass Spectrometry-Based Quantitative Proteomics

By: Michael W. Linscheid³

, Robert Ahrends³, Stefan Pieper³, Andreas Kühn³

Abstract

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During the last decades, molecular sciences revolutionized biomedical research and gave rise to the biotechnology industry. During the next decades, the application of the quantitative sciences – informatics, physics, chemistry, and engineering – to biomedical research brings about the next revolution that will improve human healthcare and certainly create new technologies, since there is no doubt that small changes can have great effects. It is not a question of “yes” or “no,” but of “how much,” to make best use of the medical options we will have.

In this context, the development of accurate analytical methods must be considered a cornerstone, since the understanding of biological processes will be impossible without information about the minute changes induced in cells by interactions of cell constituents with all sorts of endogenous and exogenous influences and disturbances.

The first quantitative techniques, which were developed, allowed monitoring relative changes only, but they clearly showed the significance of the information obtained. The recent advent of techniques claiming to quantify proteins and peptides not only relative to each other, but also in an absolute fashion, promised another quantum leap, since knowing the absolute amount will allow comparing even unrelated species and the definition of parameters will permit to model biological systems much more accurate than before. To bring these promises to life, several approaches are under development at this point in time and this review is focused on those developments.

Affiliation(s): (3) Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany

Book Title: Proteomics: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 564 | Pub. Date: Dec-01-2009 | Page Range: 189-205 | DOI: 10.1007/978-1-60761-157-8_11

Subject: Protein Science

Key Words: Isotope dilution concept - Stable-isotope labeling - Label-free quantitation - LC–/MS

References

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