SpringerProtocols: Abstract: Bacterial Artificial Chromosome Transgenesis for Zebrafish

Bacterial Artificial Chromosome Transgenesis for Zebrafish

By: Zhongan Yang⁴, Hong Jiang⁴, Shuo Lin⁴

Abstract

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Transgenesis using bacterial artificial chromosomes (BAC) allows greater fidelity in directing desirable expression of transgenes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of gene expression in a living organism. We have developed a streamlined procedure of directly selecting multiple BAC clones based on public sequence databases followed by rapid modification with green fluorescent protein or red fluorescent protein for transgenic analysis in zebrafish. In this chapter, experimental procedures for BAC DNA preparation and generation of transgenic zebrafish lines by microinjection are described.

Affiliation(s): (4) Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, 90095-1606, USA

Book Title: Zebrafish: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 546 | Pub. Date: Jan-01-2010 | Page Range: 103-116 | DOI: 10.1007/978-1-60327-977-2_7

Subject: Biochemistry

Key Words: Transgenic zebrafish - Bacterial artificial chromosomes - BAC modification - Fluorescent reporter genes - GFP/RFP.

References

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