SpringerProtocols: Abstract: Derivation of High-Purity Oligodendroglial Progenitors

5. Derivation of High-Purity Oligodendroglial Progenitors

By: Maya N. Hatch³, Gabriel Nistor³, Hans S. Keirstead³

Abstract

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Oligodendrocytes are a type of glial cells that play a critical role in supporting the central nervous system (CNS), in particular insulating axons within the CNS by wrapping them with a myelin sheath, thereby enabling saltatory conduction. They are lost, and myelin damaged – demyelination – in a wide variety of neurological disorders. Replacing depleted cell types within demyelinated areas, however, has been shown experimentally to achieve remyelination and so help restore function. One method to produce oligodendrocytes for cellular replacement therapies is through the use of progenitor or stem cells. The ability to differentiate progenitor or stem cells into high-purity fates not only permits the generation of specific cells for transplantation therapies, but also provides powerful tools for studying cellular mechanisms of development. This chapter outlines methods of generating high-purity OPCs from multipotent neonatal progenitor or human embryonic stem cells.

Affiliation(s): (3) Department of Anatomy and Neurobiology, Reeve-Irvine Research Center, 2111 Gillespie Neuroscience Research Facility, College of Medicine, University of California at Irvine, 92697-4292 Irvine, CA, USA

Book Title: Neural Cell Transplantation: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 549 | Pub. Date: Jun-01-2009 | Page Range: 59-75 | DOI: 10.1007/978-1-60327-931-4_5

Subject: Cell Biology

Key Words: High-purity oligodendrocyte precursor cultures - Oligodendrocytes - Remyelination - Oligodendrocyte precursors

References

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