SpringerProtocols: Abstract: Differentiation of Neuroepithelia from Human Embryonic Stem Cells

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4. Differentiation of Neuroepithelia from Human Embryonic Stem Cells

By: Xiaofeng Xia³, Su-Chun Zhang³

Abstract

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We describe the method for efficiently differentiating human embryonic stem cells to neuroepithelial cells in a chemically defined condition. The protocol was established based on the fundamental principle of in vivo neuroectodermal development. The temporal course, morphological transformation, and shift in gene expression of our neuroepithelial differentiation closely resemble those occur during in vivo development. In particular, the primitive neuroepithelial cells generated by this protocol can be further induced into neuronal and glial cells with forebrain, mid/hind brain, and spinal cord identities and targeted transmitter phenotypes.

Affiliation(s): (3) Departments of Anatomy and Neurology, School of Medicine and Public Health, Waisman Center, University of Wisconsin, Madison, 1500 Highland Avenue, 53705 Madison, WI, USA

Book Title: Neural Cell Transplantation: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 549 | Pub. Date: Jun-01-2009 | Page Range: 51-58 | DOI: 10.1007/978-1-60327-931-4_4

Subject: Cell Biology

Key Words: Human embryonic stem cells - Embryoid body - Neuroepithelia - Neuronal differentiation - Chemically defined condition

References

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1.	Du, Z.W. and Zhang, S.C. (2004). Neural differentiation from embryonic stem cells: which way? Stem Cells Dev. 13, 372–381.

2.	Pankratz, M.T., Li, X.J., Lavaute, T.M., Lyons, E.A., Chen, X., Zhang, S.C. (2007). Directed Neural Differentiation of hESCs via an Obligated Primitive Anterior Stage. Stem Cells. 25, 1511–1520.

3.	Li, X.J., Du, Z.W., Zarnowska, E.D., Pankratz, M., Hansen, L.O., Pearce, R.A., Zhang, S.C. (2005). Specification of motoneurons from human embryonic stem cells. Nat Biotechnol. 23, 215–221.

4.	Yan, Y., Yang, D., Zarnowska, E.D., Du, Z., Werbel, B., Valliere, C., Pearce, R.A., Thomson, J.A., Zhang, S.C. (2005). Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells. Stem Cells. 23, 781–790.

5.	Zhang, S.C. (2006). Neural subtype specification from embryonic stem cells. Brain Pathol. 16, 132–142.

6.	Zhang, S.C., Wernig, M., Duncan, I.D., Brustle, O., Thomson, J.A. (2001). In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat Biotechnol. 19, 1129–1133.

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