SpringerProtocols: Abstract: Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies

12. Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies

By: Yoichi Kondo³, Ian D. Duncan³

Abstract

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Leukodystrophies represent a wide variety of hereditary disorders of the white matter in the central nervous system, where the patients, mostly in infancy or childhood, suffer from progressive and often fatal neurological symptoms due to either a delay or lack of myelin development or loss of myelin. As only supportive therapies are available for the majority of the leukodystrophies, replacing genetically defective oligodendrocytes with intact oligodendrocytes by transplantation has a potential as a curative therapy. Animal models of leukodystrophies have been valuable in developing effective strategies of myelin repair in human diseases. This chapter discusses the animal models of leukodystrophies and describes methods for (a) derivation of mouse oligodendrocyte progenitor cells (OPCs) in vitro as a source of donor myelin-forming cells and (b) transplantation of OPCs into the brain and spinal cord of mouse models of leukodystrophies.

Affiliation(s): (3) Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, 53706 Madison, WI, USA

Book Title: Neural Cell Transplantation: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 549 | Pub. Date: Jun-01-2009 | Page Range: 175-185 | DOI: 10.1007/978-1-60327-931-4_12

Subject: Cell Biology

Key Words: Leukodystrophy - Myelin repair - Oligodendrocyte progenitor - Oligosphere - Transplantation

References

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