SpringerProtocols: Abstract: Tissue Microarrays as a Tool in the Discovery and Validation of Tumor Markers

Tissue Microarrays as a Tool in the Discovery and Validation of Tumor Markers

By: Stephen M. Hewitt¹

Abstract

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Tissue microarrays are a platform of condensed histopathology that has revolutionized the translation of basic science to clinical utility. Tissue microarrays have resulted in a paradigm shift from histopathology to immunopathology and moved analysis of small selected samples sets of tens of specimens to a high-throughput environment of hundreds of specimens. Tissue microarrays have influenced validation strategies, but have a role in discovery as well, allowing a pathways approach to analysis of tumors.

Affiliation(s): (1) Tissue Array Research Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

Book Title: Tumor Biomarker Discovery: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 520 | Pub. Date: Feb-03-2009 | Page Range: 151-161 | DOI: 10.1007/978-1-60327-811-9_11

Subject: Cancer Research

Key Words: Tissue microarray - Immunohistochemistry - Pathology - Histology - Tumor - Formalin-fixed paraffin-embedded tissue

References

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11.	Geiszt M, Lekstrom K, Brenner S, Hewitt SM, Dana R, Malech HL, Leto TL. NAD(P)H oxidase 1, a product of differentiated colon epithelial cells, can partially replace glycoprotein 91phox in the regulated production of superoxide by phagocytes. J Immunol. 2003 Jul 1;171(1):299–306.

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