SpringerProtocols: Abstract: Using RNA Interference to Study Protein Function

11. Using RNA Interference to Study Protein Function

By: Carol D. Curtis³, Ann M. Nardulli³

Abstract

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RNA interference can be extremely useful in determining the function of an endogenously-expressed protein in its normal cellular environment. In this chapter, we describe a method that uses small interfering RNA (siRNA) to knock down mRNA and protein expression in cultured cells so that the effect of a putative regulatory protein on gene expression can be delineated. Methods of assessing the effectiveness of the siRNA procedure using real time quantitative PCR and Western analysis are also included.

Affiliation(s): (3) Department of Molecular and Integrative Physiology, University of Illinois, Urbana, IL, USA

Book Title: The Nuclear Receptor Superfamily: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 505 | Pub. Date: Dec-01-2008 | Page Range: 187-204 | DOI: 10.1007/978-1-60327-575-0_11

Subject: Cell Biology

Key Words: RNA interference - siRNA - Transcription regulation - Gene silencing

References

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