SpringerProtocols: Abstract: 5. Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors

5. Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors

By: Raj Mutharasan⁵

Abstract

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In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in this chapter. The specificity of the sensor is obtained by immobilizing an antibody specific to the antigen of interest. The piezoelectric cantilever sensors are very sensitive and can easily detect a 60 kDa protein at 100 fg/mL concentration. Experimental procedure for carrying out detection of a target analyte is outlined and a sample set of results is included.

Affiliation(s): (5) Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA, USA

Book Title: Biosensors and Biodetection: Methods and Protocols: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors

Series: Methods in Molecular Biology | Volume: 504 | Pub. Date: Dec-11-2008 | Page Range: 73-82 | DOI: 10.1007/978-1-60327-569-9_5

Subject: Imaging/Radiology

Key Words: Biosensor - Fabrication - Resonant frequency - Immobilization - Antibody - Mass-change

References

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