SpringerProtocols: Abstract: 3. Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection

3. Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection

By: Jong Seol Yuk⁵, Kwon-Soo Ha⁵

Abstract

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Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x–y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases.

Affiliation(s): (5) Department of Molecular and Cellular Biochemistry and Nanobio Sensor Research Center, Kangwon National University College of Medicine, Chuncheon, Kangwon-do, Korea

Book Title: Biosensors and Biodetection

Series: Methods in Molecular Biology | Volume: 503 | Pub. Date: Nov-11-2008 | Page Range: 37-47 | DOI: 10.1007/978-1-60327-567-5_3

Subject: Imaging/Radiology

Key Words: Spectral SPR biosensor - Line scanning mode - Protein array - Mumps virus - Serodiagnosis - Resonance wavelength - PDDA

References

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