SpringerProtocols: Abstract: Clone-Based Functional Genomics

8. Clone-Based Functional Genomics

By: Annick Bleys^{2 3}, Mansour Karimi^{2 3}, Pierre Hilson^{2 3}

Abstract

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Annotated genomes have provided a wealth of information about gene structure and gene catalogs in a wide range of species. Taking advantage of these developments, novel techniques have been implemented to investigate systematically diverse aspects of gene and protein functions underpinning biology processes. Here, we review functional genomics applications that require the mass production of cloned sequence repertoires, including ORFeomes and silencing tag collections. We discuss the techniques employed in large-scale cloning projects and we provide an up-to-date overview of the clone resources available for model plant species and of the current applications that may be scaled up for systematic plant gene studies.

Affiliation(s): (2) Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Gent, Belgium
(3) Department of Molecular Genetics, Ghent University, Gent, Belgium

Book Title: Plant Systems Biology

Series: Methods in Molecular Biology | Volume: 553 | Pub. Date: Jan-01-2009 | Page Range: 141-177 | DOI: 10.1007/978-1-60327-563-7_8

Subject: Plant Sciences

Key Words: Functional genomics - recombinational cloning - clone collections - ORFeome - hairpin RNA - artificial microRNA

References

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