SpringerProtocols: Abstract: Introduction: Cell-Based Assays for High-Throughput Screening

1. Introduction: Cell-Based Assays for High-Throughput Screening

By: W. Frank An, Nicola J. Tolliday¹

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Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.

Affiliation(s): (1) Chemical Biology Platform, Broad Institute of Harvard and MIT, Cambridge, MA, USA

Book Title: Cell-Based Assays for High-Throughput Screening: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 486 | Year: 2009 | Page Range: 1-12 | DOI: 10.1007/978-1-60327-545-3_1

Subject: Biochemistry

Key Words: Assay development - Cell-based assay - High-content screening - High-throughput screening - Small-molecule screening - Target identification

References

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