Monitoring the spatial distribution of prespore and pstB cell types is a sensitive method to monitor GSK-3 and Aar activity
during Dictyostelium development. Cell-specific expression of lacZ marker genes can be readily detected using enzymatic cleavage of the substrate X-gal. This protocol describes a simple method
for β-galactosidase staining in developed Dictyostelium structures.
Affiliation(s): (3) Cardiff School of Biosciences, Cardiff University, Cardiff, Wales, UK Series: Methods in Molecular Biology | Volume: 469 | Pub. Date: Jul-01-2008 | Page Range: 33-37 | DOI: 10.1007/978-1-60327-469-2_3
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