SpringerProtocols: Abstract: Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

7. Flow Cytometric and Laser Scanning Microscopic Approaches in Epigenetics Research

By: Lorant Szekvolgyi¹, Laszlo Imre², Doan Xuan Quang Minh², Eva Hegedus², Zsolt Bacso², Gabor Szabo²

Abstract

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Our understanding of epigenetics has been transformed in recent years by the advance of technological possibilities based primarily on a powerful tool, chromatin immunoprecipitation (ChIP). However, in many cases, the detection of epigenetic changes requires methods providing a high-throughput (HTP) platform. Cytometry has opened a novel approach for the quantitative measurement of molecules, including PCR products, anchored to appropriately addressed microbeads (Pataki et al. 2005. Cytometry 68, 45–52). Here we show selected examples for the utility of two different cytometry-based platforms of epigenetic analysis: ChIP-on-beads, a flow-cytometric test of local histone modifications (Szekvolgyi et al. 2006. Cytometry 69, 1086–1091), and the laser scanning cytometry-based measurement of global epigenetic modifications that might help predict clinical behavior in different pathological conditions. We anticipate that such alternative tools may shortly become indispensable in clinical practice, translating the systematic screening of epigenetic tags from basic research into routine diagnostics of HTP demand.

Affiliation(s): (1) Institut Curie, Recombinaison et Instabilité Génétique, UMR7147 CNRS, Institut Curie, Université Pierre et Marie Curie, Paris, France
(2) Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Hungary

Book Title: Chromatin Immunoprecipitation Assays: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 567 | Pub. Date: Aug-01-2009 | Page Range: 99-111 | DOI: 10.1007/978-1-60327-414-2_7

Subject: Genetics/Genomics

Key Words: Chromatin immunoprecipitation (ChIP) - flow cytometry - ChIP-on-beads - laser scanning cytometry (LSC)

References

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