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6. A Collection of Metabolite Assays

Abstract

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This chapter contains protocols for measuring 44 different metabolites. In each case, a spectrophotometric assay and at least one fluorometric assay have been presented. In many cases, additional fluorometric procedures are given for increasing sensitivity to permit measurement of as little as a few femtomoles of material. The reader should have no difficulty designing similar adaptations of any of the methods to achieve whatever sensitivity is required. Chapter 8 may be useful for this purpose.

Book Title: Enzymatic Analysis: A Practical Guide

Series: No Series | Pub. Date: Feb-08-1993 | Page Range: 111-228 | DOI: 10.1007/978-1-60327-407-4_6

Subject: Biochemistry

References

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