SpringerProtocols: Abstract: 9. Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry

9. Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry

By: Zbigniew Darzynkiewicz¹

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For convenience the term quiescent cells will be used in this chapter to define normal or tumor cells, untreated by any specific drugs, that do not progress through the mitotic cycle for an extended period of time. The definition is purposefully wide to cover a variety of situations, often with different metabolic causes, associated with irreversible or transient cell withdrawal from the cell cycle. The even wider term, noncycling cells, will be reserved to describe, in addition to quiescent cells, cells arrested in the cycle by drugs (Table 1). (See Chapter 3 for additional discussion of cellular quiescence.)

Affiliation(s): (1) Walker Laboratory, Sloan-Kettering Institute of Cancer Research, Rye, New York

Book Title: Techniques in Cell Cycle Analysis

Series: No Series | Pub. Date: Feb-04-1987 | Page Range: 255-290 | DOI: 10.1007/978-1-60327-406-7_9

Subject: Cell Biology

References

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