SpringerProtocols: Abstract: Peptide Arrays for Enzyme Profiling

2. Peptide Arrays for Enzyme Profiling

By: Alexandra Thiele¹, Johannes Zerweck², Mike Schutkowski²

Abstract

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Enzymes are key molecules in signal transduction pathways. However, only a small fraction of more than 500 predicted human kinases, 250 proteases and 250 phosphatases is characterized so far. Peptide microarray-based technologies for extremely efficient profiling of enzyme substrate specificity emerged in the last years. Additionally, patterns of enzymatic activities could be used to fingerprint the status of cells or organisms. This technology reduces set-up time for HTS assays and allows the identification of downstream targets. Moreover, peptide microarrays enable optimization of enzyme substrates. A comprehensive overview regarding enzyme profiling using peptide microarrays is presented with special focus on assay principles.

Affiliation(s): (1) Max Planck Research Unit for Enzymology of Protein Folding, Halle, Germany
(2) JPT Peptide Technologies GmbH, Berlin, Germany

Book Title: Peptide Microarrays: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 570 | Pub. Date: Aug-01-2009 | Page Range: 19-65 | DOI: 10.1007/978-1-60327-394-7_2

Subject: Protein Science

Key Words: Enzyme - substrate specificity - kinase - protease - phosphatase - peptide microarray - spot synthesis

References

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