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4. Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors
Abstract
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
Affiliation(s): (2) Crucell Holland BV, Archimedesweg 4, 2048, 2301CA Leiden, The Netherlands
Series: Methods in Molecular Biology  |  Volume: 562  |  Pub. Date: Mar-01-2009  |  Page Range: 45-60  |  DOI: 10.1007/978-1-60327-302-2_4
Subject:  Microbiology
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