SpringerProtocols: Abstract: RNAi Using a Chitosan/siRNA Nanoparticle System: In Vitro and In Vivo Applications

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6. RNAi Using a Chitosan/siRNA Nanoparticle System: In Vitro and In Vivo Applications

By: Morten Østergaard Andersen³, Kenneth Alan Howard³, Jørgen Kjems³

Abstract

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Delivery is a key issue in development of clinically relevant RNAi therapeutics. Polymeric nanoparticles formed by self-assembly of polycations with siRNA can be used for extracellular delivery, cellular uptake and intracellular trafficking as a strategy to improve the therapeutic potential of siRNA. This chapter describes a chitosan-based nanoparticle system for in vitro and in vivo transfection of siRNA into cells. The method exploits the mucoadhesive and mucopermeable properties of this cationic polysaccharide to deliver siRNA across mucosal epithelium and provides a platform for targeting human diseases with RNAi therapeutics.

Affiliation(s): (3) Interdisciplinary Nanoscience Center (iNANO), Department of Molecular Biology, University of Aarhus, Aarhus C, Denmark

Book Title: Therapeutic Applications of RNAi: Methods and Protocols

Series: Methods in Molecular Biology | Volume: 555 | Pub. Date: Jan-01-2009 | Page Range: 77-86 | DOI: 10.1007/978-1-60327-295-7_6

Subject: Genetics/Genomics

Key Words: siRNA - Chitosan - Nanoparticles - Macrophages - Nasal Delivery - Intraperitoneal Delivery - TNFα - Freeze Drying

References

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1.	Fire, A., et al. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811.

2.	Elbashir, S. M., et al. (2001). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494–498.

3.	Pouton, C. W. and Seymour, L. W. (2001) Key issues in non-viral gene delivery. Adv. Drug Deliv. Rev. 46, 187–203.

4.	Elouahabi, A. and Ruysschaert, J. M. (2005) Formation and intracellular trafficking of lipoplexes and polyplexes. Mol. Ther. 11, 336–347.

5.	Howard, K. A., et al. (2006). RNA Interference in vitro and in vivo using a novel chitosan/siRNA nanoparticle system. Mol. Ther. 14, 476–484.

6.	Liu, X., et al. (2007). The influence of polymeric properties on chitosan/siRNA nanoparticle formulation and gene silencing. Biomaterials 28, 1280–1288.

7.	Soane, R. J., et al. (1999). Evaluation of the clearance characteristics of bioadhesive systems in humans. Int. J. Pharm. 178, 55–65.

8.	Artursson, P., Lindmark, T., Davis, S. S. and Illum, L. (1994). Effect of chitosan on the permeability of monolayers of intestinal epithelial cells (Caco-2). Pharm. Res. 11, 1358–1361.

9.	Feng, J., Zhao, L. and Yu, Q. (2004). Receptor-mediated stimulatory effect of oligochitosan in macrophages. Biochem. Biophys. Res. Commun. 317, 414–420.

10.	Howard, K. A., et al. (2009). Knockdown in peritoneal macrophages as an anti-inflammatory treatement in a murine arthritic model. Mol. Ther. 17, 162–168.

11.	Andersen, M. O., et al. (2008). Delivery of siRNA from lyophilized polymeric surfaces. Biomaterials 29, 506–512.

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