SpringerProtocols: Abstract: Production of Foreign Proteins in Tobacco Cell Suspension Culture

Production of Foreign Proteins in Tobacco Cell Suspension Culture

By: Véronique Gomord², Anne-Catherine Fitchette-Lainé², Lise-Anne Denmat², Dominique Michaud³, Loïc Faye²

Abstract

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The transfer of foreign genes to plant cells is most often performed using the biological vector Agrobacterium tumefaciens (1). This Agrobacterium-mediated introduction of cloned genes into plants has been widely used for promoter characterization using reporter genes, analysis of gene function, and protein targeting. Agrobacterium-mediated gene transfer has also been a method of choice for introducing genes that increase crop resistance to insects or viruses, or genes that encode foreign proteins of industrial or bio-pharmaceutical interest.

Affiliation(s): (2) Laboratoire des Transports Intracellulaires, Universite de Rouen, Faculte des Sciences, Mont-Saint-Aigen, France
(3) Centre de Recherche en Horticulture, Universite Laval, Quebec, Canada

Book Title: Recombinant Proteins from Plants: Production and Isolation of Clinically Useful Compounds

Series: Methods in Biotechnology | Volume: 3 | Pub. Date: Dec-24-1997 | Page Range: 155-164 | DOI: 10.1007/978-1-60327-260-5_12

Subject: Plant Sciences

References

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