Springer Protocols: Abstract: 9. PEGylated Adenovirus for Targeted Gene Therapy

9. PEGylated Adenovirus for Targeted Gene Therapy

By: Catherine R.³, O’ Riordan³, Antonius Song³

Abstract

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Bifunctional polyethylene glycol (PEG) molecules provide a novel approach to retargeting viral vectors without the need to genetically modify the vector. Modification of the surface of adenovirus with heterofunctional PEG allows further modification of the capsid with ligands. In addition, heterofunctional PEG modification ablates the normal tropism of the virus and reduces transduction of non-target tissues in vivo. Moreover, the addition of PEG chains to the surface of the virus shields antigen-binding sites, significantly reducing the susceptibility of the virus to antibody neutralization. Finally, T cell subsets from mice exposed to the PEGylated vector demonstrate a marked decrease in Th1 and Th2 responses, suggesting that PEG modification may help reduce the immune response to the vector.

Affiliation(s): (3) Genzyme Corporation, Framingham, MA

Book Title: Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors

Series: Methods in Molecular Biology | Volume: 434 | Pub. Date: Mar-07-2008 | Page Range: 133-160 | DOI: 10.1007/978-1-60327-248-3_9

Subject: Genetics/Genomics

Key Words: Adenovirus - PEGylation - targeting - FGF2 - innate immunity - adaptive immunity - antibody neutralization

References

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