Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells
By: Wei Liu3, Steven X. Hou3
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The advancement of Drosophila germline stem cell research accompanies the development of powerful new tools for genetic analysis. These include the techniques
of stem cell labeling, cell lineage tracing, mosaic mutant analysis, and gene manipulation in targeted cell populations, which
together constitute the critical methodologies in stem cell research. We discuss four such techniques: the tubulin-lacZ positive-labeling system; the positively marked mosaic lineage (PMML) method; the flipase/flipase recombination target (FLP/FRT)-based
mosaic mutant analysis; and the GAL80-based mosaic analysis with a repressible cell marker (MARCM) system.
Affiliation(s): (3) Mouse Cancer Genetics Program, National Institutes of Health, National Cancer Institute at Frederick, Frederick, MD, USA
Book Title: Germline Stem Cells
Series: Methods in Molecular Biology | Volume: 450 | Pub. Date: Apr-01-2008 | Page Range: 61-70 | DOI: 10.1007/978-1-60327-214-8_4
Subject: Cell Biology
Key Words: Drosophila germline stem cell - FLP/FRT - GAL4/UAS - GAL80 - MARCM - mosaic analysis - PMML - positive labeling
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