Springer Protocols: Abstract: Analysis of the C. elegans Germline Stem Cell Region

Analysis of the C. elegans Germline Stem Cell Region

By: Sarah L. Crittenden³, Judith Kimble³

Abstract

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We present methods for characterizing the mitotic and early meiotic regions of the Caenorhabditis elegans germline. The methods include examination of germlines in living and fixed worms, cell cycle analysis, analysis of markers, and initial characterization of mutants that affect germline proliferation.

Affiliation(s): (3) Howard Hughes Medical Institute and Department of Biochemistry, University of Wisconsin—Madison, Madison, WI

Book Title: Germline Stem Cells

Series: Methods in Molecular Biology | Volume: 450 | Pub. Date: Apr-01-2008 | Page Range: 27-44 | DOI: 10.1007/978-1-60327-214-8_2

Subject: Cell Biology

Key Words: BrdU - C. elegans - cell cycle - germline - meiosis - mitosis - proliferation - stem cells

References

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