Springer Protocols: Abstract: Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

By: Christina Tenenhaus Dann³, David L. Garbers⁴

Abstract

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The primary method for determining the function of a gene in rodents has been to make a knockout mouse through homologous recombination in embryonic stem cells. However, with the advent of RNA interference (RNAi) technology, new methods for studying gene function are now possible in a wide array of animals. We describe a protocol for knocking down a gene of interest in vivo in rats by stably expressing a short hairpin RNA (shRNA). Transgenic rats are produced using a simple and efficient procedure for transducing single-cell embryos with a lentiviral vector. The vector described is designed to result in ubiquitous expression of shRNA. Thus, it is well suited to study genes expressed specifically in male germ cells in which the predicted phenotype would be male sterility. This system has been used to generate a transgenic line with stable and heritable knockdown of the gene Deleted in Azoospermia-like (Dazl), resulting in male sterility and germline transmission of the transgene through females.

Affiliation(s): (3) Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX
(4) Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX

Book Title: Germline Stem Cells

Series: Methods in Molecular Biology | Volume: 450 | Pub. Date: Apr-01-2008 | Page Range: 193-209 | DOI: 10.1007/978-1-60327-214-8_14

Subject: Cell Biology

Key Words: Germ cell - lentivirus - one-cell embryo - rat - RNAi - shRNA - transgene

References

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