Springer Protocols: Abstract: Deriving Mouse Spermatogonial Stem Cell Lines

Deriving Mouse Spermatogonial Stem Cell Lines

By: Ilaria Falciatori³, Kate Lillard-Wetherell⁴, Zhuoru Wu⁵, F. Kent Hamra⁶, David L. Garbers⁷

Abstract

Full Text

Download PDF (382K)

In adult males, spermatogonial stem cells function to replenish developing gametes that are continuously released from the testes as mature spermatozoa. Because of their potential importance to research, medicine, industry, and conservation, numerous attempts have been made in the past to cultivate sperma-togonial stem cells in vitro. However, only recently have culture methods been established that effectively promote the proliferation of mammalian spermatogonial stem cells in vitro. We describe a simple and reproducible protocol for the derivation and maintenance of mouse spermatogonial stem cell lines that proliferate for long periods of time in culture.

Affiliation(s): (3) Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA
(4) Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA
(5) Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX, USA
(6) Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX, USA
(7) Department of Pharmacology and Cecil H. and Ida Green Center for Reproductive Biology Sciences, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA

Book Title: Germline Stem Cells

Series: Methods in Molecular Biology | Volume: 450 | Pub. Date: Apr-01-2008 | Page Range: 181-192 | DOI: 10.1007/978-1-60327-214-8_13

Subject: Cell Biology

Key Words: Germ cells - germline stem cells - mouse - spermatogonia - spermatogonial stem cells - spermatogonial stem cell culture - SSC - testis

References

Download References

1.	1. Kanatsu-Shinohara, M., Ogonuki, N., Inoue, K., et al. (2003) Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol. Reprod. 69, 612–616.

2.	2. Ryu, B. Y. , Kubota, H., Avarbock, M. R., and Brinster, R. L. (2005) Conservation of spermato-gonial stem cell self-renewal signaling between mouse and rat. Proc. Natl. Acad. Sci. U. S. A. 102, 14302–14307.

3.	3. Hamra, F. K., Chapman, K. M., Nguyen, D. M., Williams-Stephens, A. A., Hammer, R. E., and Garbers, D. L. (2005) Self renewal, expansion, and transfection of rat spermatogonial stem cells in culture. Proc. Natl. Acad. Sci. U. S. A. 102, 17430–14735.

4.	4. Hamra, F. K., Gatlin, J., Chapman, K. M., et al. (2002) Production of transgenic rats by lentivi-ral transduction of male germ-line stem cells. Proc. Natl. Acad. Sci. U. S. A. 99, 14931–14936.

5.	5. Dann, C. T., Alvarado, A. L., Hammer, R. E., and Garbers, D. L. (2006) Heritable and stable gene knockdown in rats. Proc. Natl. Acad. Sci. U. S. A. 103, 11246–11251.

6.	6. Kanatsu-Shinohara, M., Ikawa, M., Takehashi, M., et al. (2006) Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc. Natl. Acad. Sci. U. S. A. 103, 8018–8023.

7.	7. Kanatsu-Shinohara, M., Inoue, K., Lee, J., et al. (2004) Generation of pluripotent stem cells from neonatal mouse testis. Cell. 119, 1001–1012.

8.	8. Guan, K., Nayernia, K., Maier, L. S., et al. (2006) Pluripotency of spermatogonial stem cells from adult mouse testis. Nature. 440, 1199–1203.

Comments (Loading...)

Post comment/View all comments