Springer Protocols: Abstract: Preparation of Nasal Secretions for Proteome Analysis

Preparation of Nasal Secretions for Proteome Analysis

By: Begona Casado³, Paolo Iadarola⁴, Lewis K Pannell⁵

Abstract

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The determination of protein patterns in nasal secretions of healthy subjects can help in the early diagnosis of diseases such as acute sinusitis. The comparison of nasal lavage fluid collected from subjects with acute sinusitis before and after pharmacological treatment gives information about the drug effects on glandular secretions. Nasal secretions were stimulated with 1× NS (0.9% Normal Saline) and 24× NS in healthy subjects and in sinusitis subjects before and after pharmacological treatment. The nasal lavage fluid (NLF) proteins are precipitated with a solution of “acid-ethanol.” Using this solution, the high molecular weight proteins precipitate and separate from the low molecular weight proteins. The proteins are digested and the peptides are separated using a capillary liquid chromatographic system. Eluted peptides are analyzed on ESI-Q-TOF mass spectrometry instrument.

Affiliation(s): (3) Swiss Federal Institute of Technology, Zurich, Switzerland
(4) University of Pavia, Pavia, Italy
(5) University of South Alabama, Mobile, Alabama

Book Title: 2D PAGE: Sample Preparation and Fractionation

Series: Methods in Molecular Biology | Volume: 425 | Pub. Date: Jan-25-2008 | Page Range: 77-87 | DOI: 10.1007/978-1-60327-210-0_7

Subject: Protein Science

Key Words: CapLC-ESI-Q-ToF - liquid-liquid extraction - Nasal secretions - pharmacological treatment - proteomics - sample preparation - sinusitis

References

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